基于数据库筛选补中益气汤中7种黄酮类成分及其含量分析  

Content Analysis of Seven Flavonoids Compounds in Buzhong Yiqi Decoction Based on Database

作　　者：曹礼慧 徐汉明[2] 袁中文 陈朋[3] 冯森玲 CAO Li-hui;XU Han-ming;YUAN Zhong-wen;CHEN Peng;FENG Sen-ling(The Third Affiliated Hospital of Guangzhou Medical University/Guangdong Provincial Key Laboratory of Obstetrics Major Diseases,Guangzhou 510150,China;Shenzhen Hospital of Beijing University,Shenzhen 518033,China;Guangzhou University of Traditional Chinese Medicine,Guangzhou 510006,China)

机构地区：[1]广州医科大学附属第三医院/广东省产科重大疾病重点实验室,广东广州510150 [2]北京大学深圳医院,广东深圳518033 [3]广州中医药大学,广东广州510006

出　　处：《云南中医中药杂志》2022年第2期71-74,共4页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：广东省自然科学基金(2018A030310292);广东省基础与应用基础研究基金(2019A1515111109);广州医科大学附属第三医院博士启动科研基金(2019B03);广东省中医药局科研基金(20201213);广州市中医药和中西医结合科技项目(20202A010013)。

摘　　要：目的筛选补中益气汤治疗子宫脱垂7种黄酮类成分及其含量分析。方法运用TCMSP数据库筛选补中益气汤治疗子宫脱垂的关键化合物,采用HPLC法分析关键化合物的含量,Agilent SB-C_(18)柱(250mm×4.6 mm,5μm),流动相为色谱乙腈-0.05%磷酸水溶液梯度洗脱,进样量10μL,流速为1.0 mL/min,检测波长:254 nm,柱温为30℃。结果通过数据库筛选出7种黄酮类成分,包括槲皮素、山柰酚、柚皮素、异鼠李素、刺芒柄花素、川陈皮素、毛蕊异黄酮。结论建立HPLC法测定补中益气汤治疗子宫脱垂的7种黄酮类成分,该含量测定方法稳定、可靠、准确,可用于补中益气汤质量控制并指导临床用药。Objective:To analyze the content of seven flavonoids compounds in Buzhong Yiqi Decoction in the treatment of uterine prolapse patients.Methods:The key compounds of Buzhong Yiqi Decoction in the treatment of uterine prolapse were screened by using TCMSP database,and the content of key compounds was analyzed by HPLC.Agilent SB-C_(18) column was 250mm×4.6mm,5μm,and the mobile phase was acetonitrile-0.05%phosphoric acid aqueous solution gradient elution.The injection volume was 10μL and the flow rate was 1.0 mL/min.The detection wavelength was 254 nm,and the column temperature was 30℃.Results:Seven flavonoids were screened from the database,including quercetin,kaempferol,naringenin,isorhamnetin,formononetin,nobiletin and calycosin.Conclusion:A HPLC method was established for the determination of seven flavonoids in Buzhong Yiqi Decoction in the treatment of uterine prolapse.The content determination method is stable,reliable and accurate,and can be used for quality control of Buzhong Yiqi Decoction and guide clinical medication.

关 键 词：补中益气汤 TCMSP数据库 高效液相色谱法 黄酮类成分 含量分析 

分 类 号：R285.5[医药卫生—中药学]