不同预处理方式下肉桂枝叶内生细菌物种差异分析  

Analysis of Endophytic Bacteria Species in Cinnamon Leaves and Twigs Under Different Pretreatment Methods

作　　者：曾维星 程贤[1,2] 毕良武 卢言菊[1,2] 陈玉湘 赵振东[1,2] 张笮晦 Zeng Weixing;Cheng Xian;Bi Liangwu;Lu Yanju;Chen Yuxiang;Zhao Zhendong;Zhang Zuohui(Institute of Chemical Industry of Forest Products,CAF/National Engineering Lab.for Biomass Chemical Utilization/Key and Open Lab.of National Forestry and Grassland Administration on Forest Chemical Engineering/Key Lab.of Biomass Energy and Material,Nanjing Jiangsu 210042,China;Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Nanjing Forestry University,Nanjing Jiangsu 210037,China;Guangxi Gengyuan Flavor and Fragrance Co.Ltd.,Fangchenggang Guangxi 538100,China)

机构地区：[1]中国林业科学研究院林产化学工业研究所,生物质化学利用国家工程实验室,国家林业和草原局林产化学工程重点开放性实验室,江苏省生物质能源与材料重点实验室,江苏南京210042 [2]南京林业大学江苏省林业资源高效加工利用协同创新中心,江苏南京210037 [3]广西庚源香料有限责任公司,广西防城港538100

出　　处：《西南林业大学学报（自然科学）》2022年第1期23-29,共7页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家重点研发计划资助项目(2018YFD0600405)资助;中国林业科学研究院林产化学工业研究所研究团队建设创新工程项目(LHSXKQ2)资助。

摘　　要：利用基因测序技术对空白组(C)和经过生物预处理的2组(水预处理组,W;酵母液预处理组,Y)肉桂枝叶18个样本的内生菌进行基因测序。物种组成分析发现C组特有物种数为56个,W组为50个,而Y组达到了100个;3组样本中的变形菌门等丰度较高,总丰度占比达到了96%。对18个样本进行比较分析发现Y;和W;具有显著差异性。物种差异分析对比C组发现3组样本中厚壁菌门等为显著差异。对W、Y2组进行LEfSe多级物种差异判别分析发现W组中甲基杆菌门的丰度对差异效果影响最大;Y组中,厚壁菌门对组间差异存在最显著影响,同时其丰度对差异效果影响也是最大。Gene sequencing was performed on the endophytes of 18 samples of the control group(C)and the 2groups(water Proteobacteria pretreatment,W;yeast pretreatment,Y)of cinnamon leaves and twigs after biological pretreatment.The analysis of species composition found that the number of unique species in group C was 56group W was 50,and group Y reached 100;the abundance of Proteobacteria in the 3 groups of samples was higher,and the total abundance accounted for 96%.A comparative analysis of 18 samples found that Y;and W;have significant differences.Species difference analysis and comparison of group C found that Firmicutes among the 3groups were significantly different.The LEfSe multi-level discriminant analysis of species differences between the W and Y groups found that the abundance of Methylobacterium in the W group had the greatest impact on the difference;in the Y group,Firmicutes had the most significant difference between the groups.At the same time its abundance has the greatest impact on the difference effect.

关 键 词：肉桂枝叶 基因测序技术 生物预处理 内生菌 差异分析 

分 类 号：S718.83[农业科学—林学]