香蕉褐缘灰斑病菌检测技术研究进展  

Advances on detection techniques of the banana Sigatoka pathogen

作　　者：漆艳香[1] 谢艺贤[1] 彭军[1] 曾凡云 张欣 Qi Yanxiang;Xie Yixian;Peng Jun;Zeng Fanyun;Zhang Xin(Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Pests Detection and Control for Tropical Agriculture,Haikou 571101,China)

机构地区：[1]中国热带农业科学院环境与植物保护研究所/海南省热带农业有害生物检测监控重点实验室,海南海口571101

出　　处：《植物检疫》2022年第1期1-8,共8页Plant Quarantine

基　　金：海南省基础与应用基础研究计划(自然科学领域)高层次人才项目(2019RC278);财政部和农业农村部:国家现代农业产业技术体系(CARS-31-07)。

摘　　要：香蕉褐缘灰斑病严重影响香蕉产量和品质,是香蕉生产上的重要生物灾害之一。香蕉褐缘灰斑病的早期诊断、检疫及防治的重要基础与前提是建立快速、灵敏的检测技术,从而准确及时鉴定出病原菌种类。本文介绍了香蕉褐缘灰斑病菌的种类和分类,概述了国内外香蕉褐缘灰斑病菌检测方法的研究进展,总结和归纳了传统、血清学、DNA指纹图谱、常规PCR、双重PCR、实时荧光定量PCR、LAMP及SPR免疫传感器等检测技术在该病病原菌检测中的应用,并比较分析了各种技术的特点,以期为选择合适的香蕉褐缘灰斑病菌检测方法提供参考。Banana Sigatoka disease is one of the most important biological disasters reducing banana yield and fruit quality.Pathogen identification based on fast,sensitive detection methods is crucial to the early diagnosis,quarantine and control of this disease.In this paper,species and classification of banana Sigatoka pathogens and recent advances in detection techniques were reviewed.The detection techniques based on tradition,serology,DNA fingerprinting,routine polymerase chain reaction(PCR),duplex PCR,real-time fluorescence quantitative PCR,loop-mediated isothermal amplification(LAMP),surface plasmon resonance(SPR)immunosensor and hyperspectral images were summarized.Furthermore,the pros and cons of these methods were analyzed so as to provide useful reference for the development of new detection method of banana Sigatoka pathogen.

关 键 词：香蕉褐缘灰斑病 香蕉生球腔菌 斐济球腔菌 芭蕉球腔菌 检测 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治]