染色体外环形DNA研究进展及其在畜禽育种中的应用  

Advances in Extrachromosomal Circular DNA and Their Application in Domestic Animal Breeding

作　　者：曹修凯 王珊[2] 葛玲 张卫博 孙伟[1,2] CAO Xiu-kai;WANG Shan;GE Ling;ZHANG Wei-bo;SUN Wei(Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China,Yangzhou University,Yangzhou 225009;College of Animal Science and Technology,Yangzhou University,Yangzhou 225009)

机构地区：[1]扬州大学教育部农业与农产品安全国际联合研究实验室,扬州225009 [2]扬州大学动物科学与技术学院,扬州225009

出　　处：《生物技术通报》2022年第1期247-257,共11页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31872333);江苏省重点研发计划(现代农业)项目(BE2018354);江苏省农业重大新品种创制项目(PZCZ201739);江苏省农业科技自主创新项目(CX(18)2003)。

摘　　要：染色体外环形DNA(extrachromosomal circular DNA,eccDNA)指来源于基因组DNA并游离于染色体之外的双链环状DNA分子,它在真核生物中普遍存在。eccDNA在数目、序列长度和基因组来源上存在很大差异,其功能也不尽相同,包括端粒可变延长、rDNA拷贝数维持、衰老、耐药性和肿瘤发生等,eccDNA与畜禽表型相关性已有报道。本文综述了eccDNA的分类、产生机制、功能研究和鉴定方法等,并就eccDNA在动物育种中的应用进行阐述。Extrachromosomal circular DNA(eccDNA),exiting as double-stranded circular DNA,arise from and are outside of chromosome.They are common in eukaryote,but have strong heterogeneity in count,length,origin,which lead to functional diversity,including telomere prolonging,rDNA copy number maintenance,aging,drug resistance,tumorigenesis.The associations between eccDNA and domestic animal phenotypes have been reported as well.Here,we review the current knowledge related to eccDNA classification,biogenesis,functions,and identification,and discuss some possible future directions of eccDNA applications in animal breeding.

关 键 词：eccDNA ecDNA 高通量测序 分子标记 动物育种 

分 类 号：S813[农业科学—畜牧学]