BnaYUCCA10基因在不同油酸含量油菜中的表达  

Expression of BnaYUCCA10 gene in Brassica napus with different oleic acid content

作　　者：郭诗芬 钟婷婷 卢文斌 刘忠松[1] 邬贤梦[1,2] 官春云 肖钢[1,2] GUO Shifen;ZHONG Tingting;LU Wenbin;LIU Zhongsong;WU Xianmeng;GUAN Chunyun;XIAO Gang(College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China;Hunan Provincial Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance,Changsha,Hunan 410128,China)

机构地区：[1]湖南农业大学农学院,湖南长沙410128 [2]水稻油菜抗病育种湖南省重点实验室,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2022年第1期22-26,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：“十三五”国家重点研发项目(2016YFD0100500)。

摘　　要：为明晰油菜BnaYUCCA10基因与油酸含量之间的关系,以miRNA测序得到的BnaYUCCA10基因为对象,采用实时荧光定量PCR(RT–PCR)技术研究BnaYUCCA10基因在30份不同油酸含量的甘蓝型油菜不同部位和生长时期的表达情况,并对BnaYUCCA10基因是否参与油酸的代谢进行分析。结果表明:营养生长期大部分油菜材料的BnaYUCCA10基因表达量随生育进程逐渐增加,高油酸材料与非高油酸材料间无显著差异;在开花1 d的花中的表达量高于花蕾和开花5 d的花中的表达量,且在高油酸材料与非高油酸材料中的表达量差异显著;在角果期的表达量随生育进程逐渐降低,在高油酸材料与非高油酸材料中的表达量差异显著;BnaYUCCA10基因在角果期及开花5 d的花中的表达量与油酸含量呈极显著正相关,在高油酸材料中的表达量显著高于非高油酸材料的。推测BnaYUCCA10基因可能参与了种子中的油酸代谢,可为油菜油酸调控机制的研究提供参考依据。In order to clarify the relationship between the BnaYUCCA10 gene expression and the oleic acid content in Brassica napus,we used miRNA sequencing and real-time fluorescent quantitative PCR(RT-PCR)methods in this study to analyze the expression of BnaYUCCA10 gene in 30 samples collected from different tissues and growth periods of B.napus with different oleic acid content.And whether the oleic acid is involved in the metabolism was also analyzed.The results showed that the expression level of BnaYUCCA10 gene increased gradually with the growth process in most B.napus materials during the vegetative growth stage,and there was no significant difference between high oleic acid materials and non-high oleic acid materials.The expression level of 1 DAF flowers was higher than that of buds and 5 DAF flowers,and the expression level of high oleic acid materials and non-high oleic acid materials was significantly different.The expression level in silique stage decreased gradually with the growth process,and the expression level of high oleic acid materials and non-high oleic acid materials was significantly different.The expression level of the BnaYUCCA10 gene in silique stage and 5 DAF flowers was significantly related to the oleic acid content.The expression level of the high oleic acid materials was significantly higher than that in non-high oleic acid materials.It was suggested that BnaYUCCA10 gene might be involved in oleic acid metabolism in seeds,which could be of insight to the further research on the regulation mechanism of oleic acid in rapeseed.

关 键 词：甘蓝型油菜 油酸代谢 吲哚–3–丙酮酸单氧合酶基因 基因表达 

分 类 号：S565.401[农业科学—作物学]