富贵草叶斑病病原的鉴定  

Identification of pathogen causing Colletotrichum liriopes leaf spot on Pachysandra terminalis

作　　者：钟杰[1] 尹秀娟 钟双玉 陈锦[2] 朱俊子 李晓刚[1] ZHONG Jie;YIN Xiujuan;ZHONG Shuangyu;CHEN Jin;ZHU Junzi;LI Xiaogang(College of Plant Protection,Hunan Agricultural University,Changsha,Hunan 410128,China;Institute of Agro-Environment and Ecology,Hunan Academy of Agricultural Sciences,Changsha,Hunan 410125,China)

机构地区：[1]湖南农业大学植物保护学院,湖南长沙410128 [2]湖南省农业科学院农业环境生态研究所,湖南长沙410125

出　　处：《湖南农业大学学报（自然科学版）》2022年第1期60-64,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省自然科学基金项目(2018JJ3229)。

摘　　要：2019年5月,在湖南长沙地区富贵草上发生了一种不明原因的叶部病害,对其进行病原菌分离、形态学鉴定和科赫氏法则验证,并通过对核糖体转录间区rDNA(ITS)、肌动蛋白基因(ACT)、几丁质合成酶1基因(CHS1)和甘油醛–3–磷酸脱氢酶基因(GAPDH)序列的系统进化树分析,对病原菌进行分子鉴定,确定病原菌为冬麦刺盘孢(Colletotrichum liriopes)。In this study,we isolated and identified the pathogen of a unknown leaf spot disease occurred on Pachysandra terminalis(P.terminalis)plants,in May 2019 in Changsha,Hunan Province of China.Based on morphological characteristics,pathogenicity test,and molecular identification which was conducted by constructing the phylogenetic tree using combined gene sequences of internal transcribed spacer(ITS)rDNA,actin(ACT),chitin synthase 1(CHS1)and glyceraldehyde-3-phosphate dehydrogenase(GAPDH).Based on the above results,we can conclude that the leaf spot disease of P.terminalis was caused by Colletotrichum liriopes.

关 键 词：富贵草 叶斑病 冬麦刺盘孢 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]