生物气溶胶监测仪的校准方法比较  

Comparison of Biological Calibration Methods of Bioaerosol Monitor

作　　者：田莹 张国城 吴丹 刘佳琪 李晶晶 霍胜伟 沈上圯 潘一廷 TIAN Ying;ZHANG Guo-cheng;WU Dan;LIU Jia-qi;LI Jing-jing;HUO Sheng-wei;SHEN Shang-yi;PAN Yi-ting(National Quality Supervision and Inspection Center for Eco-environmental Products,Beijing Institute of Metrology,Beijing 100029,China;College of Environmental Sciences and Engineering,Peking University,Beijing 100871,China)

机构地区：[1]北京市计量检测科学研究院国家生态环境监测治理产品质量监督检验中心,北京100029 [2]北京大学环境科学与工程学院,北京100871

出　　处：《计量学报》2022年第1期140-144,共5页Acta Metrologica Sinica

基　　金：国家市场监管总局科技计划项目(2021MK003)。

摘　　要：生物气溶胶监测仪利用荧光光谱法检测空气中的生物性物质,对疾病防控以及生物防恐预警意义重大。研究发现目前的国产生物气溶胶监测仪在采集空气中的生物颗粒时并不能区分不同细菌状态,得到的为总细菌数。通过实时定量PCR法和菌落计数法检测BioSampler采集瓶中的微生物,发现总细菌数和可培养的细菌数差异显著。利用扫描电子显微镜和荧光显微镜观察发现,采样器的撞击和离心作用影响了所收集的空气中微生物的生存能力,因此不宜用菌落计数法校准生物气溶胶监测仪。The bioaerosol monitor detect biological substances in the air based on laser-induced fluorescence,which is of great significance for disease prevention and early warning of biological terrorism.The study found that the current domestic bioaerosol monitor can t distinguish different bacterial states when collecting biological particles in the air.The bioaerosol monitor detect the total number of bacteria.Through real-time quantitative PCR and colony counting method to detect the microorganisms in the BioSampler,it was found that the total number of bacteria and the number of cultivable bacteria were significantly different.Observation by scanning electron microscope and fluorescence microscope found that the impact and centrifugation of the sampler affected the viability of the microorganisms,so it is not suitable to calibrate the bioaerosol monitor by the colony culture method.

关 键 词：计量学 生物气溶胶监测仪 菌落计数 实时定量PCR 荧光显微镜 扫描电子显微镜 

分 类 号：TB99[一般工业技术—计量学]