基于Akt/mTOR信号通路探讨药熨疗法对神经根型颈椎病大鼠神经细胞结构及自噬因子表达的影响  

Discussion of effects of hot medicinal compress therapy on nerve cell structure and expression of autophagy factor in rats with cervical spondylotic radiculopathy based on Akt/mTOR pathway

作　　者：赵玉玲 黄沂[1] 周艳琼[1] 蒋菲菲[1] 陈海燕[1] ZHAO Yuling;HUANG Yi;ZHOU Yanqiong;JIANG Feifei;CHEN Haiyan(Department of Nursing,the First Affiliated Hospital of Guangxi University of Chinese Medicine,Guangxi Zhuang Autonomous Region,Nanning 530023,China)

机构地区：[1]广西中医药大学第一附属医院护理部,广西南宁530023

出　　处：《中国医药导报》2022年第3期4-8,共5页China Medical Herald

基　　金：广西医疗卫生适宜技术开发与推广应用项目(S2017047);广西中医药大学校级科研项目(2020MS020);中华护理学会立项科研课题(ZHKY201924)。

摘　　要：目的基于Akt/mTOR通路探讨药熨疗法对神经根型颈椎病(CSR)大鼠神经细胞超微结构及自噬因子表达的影响。方法选取4周龄SPF级SD大鼠18只,体重150~200 g,随机选取其中6只作为空白组,其余建立CSR模型。造模成功后,将其按随机数字表法分为模型组、药熨组,每组各6只。空白组、模型组不予任何处理,正常饲养。药熨组选取膀胱经、督脉、胆经进行中药药熨,连续7 d。实验结束后,采用HE染色观察神经细胞结构;透射电子显微镜观察神经细胞超微结构;Western blot检测p-Akt、p-mTOR、Beclin-1、LC3-Ⅱ/LC3-Ⅰ蛋白表达水平。结果HE染色可见空白组神经根组织形态基本正常。模型组组织受损较严重,神经根外膜包裹不完整,纤维排列紊乱,部分纤维轴突消失;胞体数量明显减少,核仁不明显。药熨组组织形态部分受损,神经根外膜包裹较完整,纤维排列较整齐,间质有轻度炎症细胞浸润。透射电子显微镜下可见空白组细胞胞质丰富,各细胞器形态正常。模型组细胞核有空洞,线粒体膜模糊,部分嵴断裂,胞质内见大量溶酶体与自噬小体。药熨组细胞质丰富,线粒体部分膜破裂,部分嵴断裂溶解,胞质内见溶酶体与自噬小体。模型组p-Akt、p-mTOR蛋白表达低于空白组,LC3-Ⅱ/LC3-Ⅰ、Beclin-1高于空白组,差异有高度统计学意义(P<0.01)。药熨组p-Akt、p-mTOR蛋白表达高于模型组,LC3-Ⅱ/LC3-Ⅰ、Beclin-1低于模型组,差异有高度统计学意义(P<0.01)。结论药熨疗法可能通过提高p-Akt、p-mTOR蛋白表达激活Akt/mTOR通路发挥抑制细胞过度自噬、减少细胞凋亡、促进受损神经根复原的作用。Objective To explore the effects of hot medicinal compress therapy on neuronal ultrastructure and expression of autophagy factors in rats with cervical spondylotic radiculopathy(CSR)based on Akt/mTOR signaling pathway.Methods A total of 184-week-old SPF SD rats,weighing 150-200 g,and six of them were randomly selected as a blank group,and the rest were established as CSR models.After the molding was successful,they were divided into model group and hot medicinal compress group according to random number table method,with six rats in each group.Blank group and model group were fed normally without any treatment.In the hot medicinal compress group,the bladder channel,the governor channel,and the gallbladder channel were ironed for seven days.After the experiment,the structure of nerve cells was observed by HE staining.Transmission electron microscopy was used to observe the ultrastructure of nerve cells.The expression levels of p-Akt,p-mTOR,Beclin-1,and LC3-Ⅱ/LC3-Ⅰproteins were detected by Western blot.Results HE staining showed that the tissue morphology of nerve root in the blank group was basically normal.In the model group,the tissue was severely damaged,the outer membrane of nerve root was incomplete,the fibers were disordered,and the axons of some fibers disappeared.The number of cell bodies decreased obviously,but nucleoli were not obvious.In the hot medicinal compress group,the tissue morphology was partially damaged,the nerve root outer membrane was more complete,the fibers were arranged more neatly,and the interstitium was infiltrated with mild inflammatory cells.Transmission electron microscopy showed that cells in blank group had abundant cytoplasm and normal organelles.In the model group,there were nuclear cavities,mitochondrial membrane was fuzzy and some cristae was broken,many lysosomes and autophagosomes were found in the cytoplasm.In the hot medicinal compress group,cytoplasm was abundant,part of mitochondrial membrane was broken,part of cristae was broken and dissolved,lysosomes and autophago

关 键 词：Akt/mTOR通路 药熨疗法 神经根型颈椎病 自噬 

分 类 号：R244[医药卫生—针灸推拿学]