不同频率电针对脑缺血再灌注模型大鼠的影响  

Effects of different frequencies electro-acupuncture on cerebral ischemia reperfusion model rats

作　　者：廖恒 范荣国[2] 李又春 高玉姣 穆敬平 LIAO Heng;FAN Rongguo;LI Youchun;GAO Yujiao;MU Jingping(Sleep Psychosomatic Medicine Center,Taihe Hospital of Shiyan City Affiliated Hospital of Hubei University of Medicine,Hubei Province,Shiyan 442000,China;Department of Health Management,Danjiangkou First Hospital,Hubei Province,Danjiangkou 442700,China)

机构地区：[1]湖北省十堰市太和医院湖北医药学院附属医院睡眠心身医学中心,湖北十堰442000 [2]湖北省丹江口市第一医院健康管理科,湖北丹江口442700

出　　处：《中国医药导报》2022年第3期9-12,23,共5页China Medical Herald

基　　金：湖北省卫生健康委科研项目(ZY2021M006)。

摘　　要：目的观察不同频率电针对脑缺血再灌注模型大鼠的影响。方法选取雄性SPF级SD大鼠50只,体重(235±5)g,随机选取其中10只作为假手术组,其余采用大脑中动脉闭塞法建立脑缺血再灌注模型,造模成功后采用随机数字表法将其分为模型组和高、中、低频电针组,每组10只。高(100 Hz)、中(50 Hz)、低(2 Hz)频电针组以百会、神庭、水沟进行电针治疗,共干预4周,假手术组和模型组不予干预。4周后,比较五组造模后及干预1~4周的局部脑血流(rCBF)、血液流变学指标、红细胞渗透脆性(EOF)、血脑屏障通透性(脑组织中伊文思蓝含量),以及糖水偏好实验、Morris水迷宫试验结果。结果模型组血液黏滞度(高、中、低切)、血浆黏度、红细胞聚集指数、脑组织伊文思蓝含量均高于假手术组,糖水偏爱率、EOF(最大、最小)均低于假手术组,逃避潜伏期长于假手术组,穿台次数少于假手术组,差异有统计学意义(P<0.05)。高、中、低频电针组血液黏滞度(高、中、低切)、血浆黏度、红细胞聚集指数、脑组织伊文思蓝含量均低于模型组;糖水偏爱率、EOF(最大、最小)均高于模型组,逃避潜伏期短于模型组,穿台次数多于模型组,差异有统计学意义(P<0.05)。低频电针组血液黏滞度(高、中、低切)、血浆黏度、红细胞聚集指数、脑组织伊文思蓝含量均低于高、中频电针组;糖水偏爱率、EOF(最大、最小)均高于高、中频电针组,逃避潜伏期短于高、中频电针组,穿台次数多于高、中频电针组,差异有统计学意义(P<0.05)。模型组及高、中、低频电针组组内各时间点rCBF比较,差异有统计学意义(P<0.05)。模型组及高、中、低频电针组组内各时间点比较,差异有统计学意义(P<0.05)。模型组各时间点的rCBF均低于同期假手术组,高、中、低频电针组干预1~4周的rCBF均高于同期模型组,低频电针组干预1~4周的rCBF均高于同期高、Objective To observe the effects of different frequencies electro-acupuncture on cerebral ischemia reperfusion model rats.Methods A total of 50 SPF male SD rats with the body weight of(235±5)g were selected,and ten of them were randomly selected as the sham operation group.The rest were established by middle cerebral artery occlusion to establish cerebral ischemia reperfusion model.After the molding was successful,they were divided into model group and high,medium,low frequency electro-acupuncture groups by random number table method,with ten rats in each group.High(100 Hz),medium(50 Hz),and low(2 Hz)frequency electro-acupuncture groups were treated with electro-acupuncture at bǎihuì,shéntíng,and shuǐgōu,a total of four weeks of intervention,sham operation group and model group did not intervene.After four weeks,regional cerebral blood flow(rCBF),hemorheological indexes,osmotic fragility of erythrocyte(EOF),blood-brain barrier permeability(Evans blue content in brain tissue),sugar water preference test,and Morris water maze test were compared among five groups.Results In the model group,the whole blood viscosity(high,medium,low cut),plasma viscosity,red blood cell aggregation index,Evans blue content of brain tissue were all higher than those in sham operation group,the sugar water preference rate,EOF(maximum and minimum)were lower than those in the sham operation group,and the escape latency was longer than that in the sham operation group,and the number of table crossing was lower than that in the sham operation group,and the differences were statistically significant(P<0.05).In high,medium,low frequency electro-acupuncture groups,whole blood viscosity(high,medium,low cut),plasma viscosity,red blood cell aggregation index,Evans blue content of brain tissue were all lower than those in model group,the sugar water preference rate,EOF(maximum and minimum)were higher than those in the model group,and the escape latency was shorter than that in the model group,and the number of table crossing was higher than

关 键 词：电针 频率 脑缺血再灌注 血脑屏障 神经功能 学习记忆能力 

分 类 号：R245[医药卫生—针灸推拿学]