支气管哮喘患儿外周血中miR-138表达与Th1/Th2平衡的关系  

Relationship between miR-138 expression and Th1/Th2 balance in peripheral blood of children with bronchial asthma

作　　者：廖全辉[1] 王水蓉 黄龙[1] 蔡雪 付天明[2] 李科兴 LIAO Quanhui;WANG Shuirong;HUANG Long;CAI Xue;FU Tianming;LI Kexing(Department of Paediatrics,Longquanyi District of Chengdu Maternity and Child Health Care Hospital,Sichuan Province,Chengdu 610100,China;Department of Obstetrics and Gynecology,Longquanyi District of Chengdu Maternity and Child Health Care Hospital,Sichuan Province,Chengdu 610100,China;Division of Neonatology,Longquanyi District of Chengdu Maternity and Child Health Care Hospital,Sichuan Province,Chengdu 610100,China)

机构地区：[1]成都市龙泉驿区妇幼保健院儿科,四川成都610100 [2]成都市龙泉驿区妇幼保健院妇产科,四川成都610100 [3]成都市龙泉驿区妇幼保健院新生儿科,四川成都610100

出　　处：《中国医药导报》2022年第3期87-90,98,共5页China Medical Herald

基　　金：四川省卫生和计划生育委员会科研课题(17PJ161)。

摘　　要：目的探讨支气管哮喘(BA)患儿外周血中微小RNA(miRNA)-138表达与辅助性T细胞(Th)1/Th2平衡的关系。方法选取成都市龙泉驿区妇幼保健院2020年1月至2021年3月收治的103例BA患儿,根据临床表现分为急性发作期组(42例),临床缓解期组(61例),另选取同期82名体检健康儿童为对照组。qRT-PCR检测外周血miR-138、T-bet、GATA结合蛋白3(GATA3)表达;流式细胞检测外周血Th1、Th2百分比,酶联免疫吸附试验法测定外周血白细胞介素-2(IL-2)、IL-4、IL-10、γ干扰素(INF-γ)水平。结果急性发作期组外周血miR-138表达、Th2百分比高于临床缓解期组、对照组,Th1百分比、Th1/Th2低于临床缓解期组、对照组(P<0.05);临床缓解期组外周血miR-138表达、Th2百分比高于对照组,Th1百分比、Th1/Th2低于对照组(P<0.05)。急性发作期组外周血T-bet水平低于临床缓解期组、对照组,GATA3水平高于临床缓解期组、对照组(P<0.05);临床缓解期组外周血T-bet水平低于对照组,GATA3水平高于对照组(P<0.05)。急性发作期组外周血IL-2、IFN-γ水平低于临床缓解期组、对照组,IL-4、IL-10水平高于临床缓解期组、对照组(P<0.05);临床缓解期组外周血IL-2、IFN-γ水平低于对照组,IL-4、IL-10水平高于对照组(P<0.05)。BA患儿外周血miR-138表达与T-bet、Th1/Th2、IL-2、IFN-γ呈负相关(r=-0.498、-0.515、-0.464、-0.428,P<0.01),与GATA3、IL-4、IL-10呈正相关(r=0.595、0.518、0.386,P<0.01)。结论BA患儿外周血miR-138表达显著上调,可能通过调节Th1/Th2平衡介导炎症反应,参与BA发生发展。Objective To investigate the relationship between microRNA(miRNA)-138 expression and T helper cell(Th)1/Th2 balance in peripheral blood of children with bronchial asthma(BA).Methods A total of 103 children with BA admitted to Longquanyi District of Chengdu Maternity and Child Health Care Hospital from January 2020 to March 2021 were selected.According to clinical manifestations,they were divided into acute attack group(42 cases)and clinical remission group(61 cases),and 82 healthy children with physical examination were selected as control group during the same period.Peripheral blood miR-138,T-bet,GATA binding protein 3(GATA3)detected by qRT-PCR,peripheral blood Th1 percentage and Th2 percentage detected by flow cytometry,and peripheral blood interleukin-2(IL-2),IL-4,IL-10,and interferon-γ(INF-γ)levels were detected by enzyme linked immunosorbent assay.Results MiR-138 expression and Th2 percentage in peripheral blood of acute attack group were higher than those of clinical remission group and control group,while Th1 percentage and Th1/Th2 were lower than those of clinical remission group and control group(P<0.05);MiR-138 expression and Th2 percentage in peripheral blood in clinical remission group were higher than those in control group,while Th1 percentage and Th1/Th2 were lower than those in control group(P<0.05).T-bet level in peripheral blood of acute attack group was lower than that of clinical remission group and control group,while GATA3 level was higher than that of clinical remission group and control group(P<0.05);T-bet level in peripheral blood of clinical remission group was lower than that of control group,and GATA3 was higher than that of control group(P<0.05).The levels of IL-2 and IFN-γin peripheral blood of acute attack group were lower than those of clinical remission group and control group,while the levels of IL-4 and IL-10 were higher than those of clinical remission group and control group(P<0.05);the levels of IL-2 and IFN-γin peripheral blood in clinical remission group were lower tha

关 键 词：支气管哮喘 微小RNA-138 辅助性T细胞 炎症 

分 类 号：R562[医药卫生—呼吸系统]