脑蛋白水解物-Ⅰ防治C57小鼠脑衰老的作用机制  

Effect and mechanism of cerebroprotein hydrolysate-Ⅰfor preventing and treating brain senescence in C57 mice

作　　者：朱琳 任宇倩 倪钦帅 李义召 任雷鸣[3] 郭云良 ZHU Lin;REN Yu-Qian;NI Qin-Shuai(Institute of Integrative Medicine,Qingdao University Medical College,Qingdao 266021,Shandong,China)

机构地区：[1]青岛大学医学部中西医结合中心,山东青岛266021 [2]济南房干康复医院 [3]河北医科大学中西医结合研究所

出　　处：《中国老年学杂志》2022年第4期892-897,共6页Chinese Journal of Gerontology

基　　金：国家自然科学基金项目资助(81973501);河北智同科技计划项目(2019ZD1901)。

摘　　要：目的研究脑蛋白水解物(CH)-Ⅰ延缓D-半乳糖(D-gal)致C57小鼠衰老的作用机制。方法C57/BL6N小鼠随机分为对照组、模型组、CH-Ⅰ低剂量组和CH-Ⅰ高剂量组,皮下注射D-gal以诱导衰老模型,给药组腹腔注射CH-Ⅰ。Morris水迷宫实验检测小鼠的学习和记忆能力,苏木素-伊红(HE)染色观察神经元的形态结构,Western印迹检测脑源性神经营养因子(BDNF)、信号传感器和转录激活因子(STAT)3/磷酸化(p)-STAT3和端粒酶逆转录酶(TERT)的表达,PCR-酶联免疫吸附试验(ELISA)检测端粒酶活性的表达。结果与对照组相比,模型组学习记忆能力显著下降(P<0.01),海马神经元损伤明显增加(P<0.01),端粒酶活性明显降低(P<0.01),BDNF、TERT和p-STAT3表达水平下降(P<0.01)。而给予不同浓度的CH-Ⅰ预处理后,衰老小鼠的学习记忆能力障碍得到改善(P<0.05),海马神经元损伤显著下降(P<0.05),并且CH-Ⅰ低、高浓度均可以显著提高小鼠海马组织的端粒酶活性(P<0.05),上调BDNF、端粒酶催化亚基TERT和调控因子STAT3的蛋白表达(P<0.05)。结论CH-Ⅰ可以通过提高端粒酶活性的表达从而减少D-gal衰老小鼠海马组织的衰老损伤。Objective To investigate the protective effect of cerebroprotein hydrolysate(CH)-Ⅰon brain aging injury induced by D-galactose(D-gal)in C57 mice.Methods C57/BL6N mice were randomly divided into control group,model group,CH-Ⅰlow-dose group and CH-Ⅰhigh-dose group.C57/BL6N mice were given subcutaneous injection of D-gal to establish senescence models,and the administration group was intraperitoneally injected with CH-Ⅰ.Morris water maze test was used to detect the learning and memory ability of mice.The morphology of neurons was detected by hematoxylin-eosin(HE).The expressions of brain-derived neurotrophic factor(BDNF),STAT3/p-STAT3 and telomerase reverse transcriptase(TERT)in the hippocampal region were determined by Western blot.Telomerase activity was detected by PCR-ELISA.Results Compared with control group,learning and memory ability of mice in model group were significantly decreased(P<0.01),damage of hippocampal neurons was significantly increased(P<0.01),telomerase activity was significantly decreased(P<0.01),and expression levels of BDNF,TERT,p-STAT3 were significantly decreased(P<0.01).After pretreatment with different concentrations of CH-Ⅰ,learning and memory ability of aging mice were significantly improved(P<0.05),damage of hippocampal neurons was significantly decreased(P<0.05).Telomerase activity in mouse hippocampus was significantly increased by low and high concentrations of CH-Ⅰ(P<0.05),the expression of BDNF,TERT and p-STAT3 were significantly increased(P<0.05).Conclusions CH-Ⅰcould reduce the aging damage of hippocampal tissues in D-gal aging mice by increasing the expression of telomerase activity.

关 键 词：脑蛋白水解物-Ⅰ D-半乳糖 衰老 端粒酶 

分 类 号：Q255[生物学—细胞生物学]