化痰除湿汤对衰老大鼠胸腺皮质超微结构和氧化损伤的影响  

Effect of Huatan Chushi Decoction on thymus cortex ultrastructure and oxidative damage in aging rats

作　　者：张丽芬 田曜恺 ZHANG Li-Fen;TIAN Yao-Kai(Department of Nephrology,Third Affiliated Hospital,Beijing University of Chinese Medicine,Beijing 100029,China;Second Clinical Medical School,Guangzhou University of Chinese Medicine,Guangzhou 510006,China)

机构地区：[1]北京中医药大学第三附属医院肾病科,北京100029 [2]广州中医药大学第二临床医学院,广州510006

出　　处：《中华老年多器官疾病杂志》2022年第1期49-54,共6页Chinese Journal of Multiple Organ Diseases in the Elderly

摘　　要：目的研究化痰除湿汤对衰老大鼠胸腺皮质超微结构和氧化损伤的作用。方法选择体质量130~170 g Wistar大鼠40只,随机分为4组(n=10):正常对照组为颈背皮下注射1 ml/(kg·d)生理盐水,灌胃10 ml/(kg·d)蒸馏水;衰老组为颈背皮下注射D-半乳糖溶液48 mg/(kg·d),灌胃10 ml/(kg·d)蒸馏水;化痰除湿组为颈背皮下注射D-半乳糖溶液48 mg/(kg·d),灌胃化痰除湿汤水煎剂20 g/(kg·d);阳性对照组为颈背皮下注射D-半乳糖溶液48 mg/(kg·d),灌胃维生素E溶液0.3 g/(kg·d)。共干预40 d。完成干预后,颈椎脱臼法处死大鼠,检测血清总抗氧化力(T-AOC)、乳酸脱氢酶(LDH)及脂质过氧化物(LPO)含量;透射电镜观察胸腺皮质上皮细胞和淋巴细胞超微结构。采用SPSS 20.0统计软件进行数据分析。多组间比较采用单因素方差分析(One-way ANOVA),组间两两比较采用Bonferroni检验。P<0.05为差异有统计学意义。结果实验14、28、41 d各组大鼠体质量均有明显增长,化痰除湿组体质量增长率较其他3组略低,但各组间比较,差异均无统计学意义(均P>0.05)。治疗第41天,与正常对照组比较,衰老组大鼠血清T-AOC水平显著降低,血清LDH、LPO水平显著升高,差异均有统计学意义(均P<0.01)。与衰老组比较,化痰除湿组和阳性对照组血清T-AOC水平均升高,血清LDH水平显著下降,差异均有统计学意义(均P<0.05);化痰除湿组血清LPO水平显著下降,差异有统计学意义(P<0.01);阳性对照组血清LPO水平也有降低,但差异无统计学意义(P>0.05)。与阳性对照组比较,化痰除湿组血清T-AOC水平显著较高,血清LPO水平显著较低,差异均有统计学意义(均P<0.01);2治疗组血清LDH比较,差异无统计学意义(P>0.05)。衰老组胸腺皮质中上皮细胞和淋巴细胞明显减少,细胞结构破坏明显,细胞核皱缩变形,胞质内线粒体和内质网等细胞器明显减少,溶酶体增多;化痰除湿组和阳性对照组胸腺皮质中上皮细�Objective To study the effect of Huatan Chushi Decoction on the ultrastructure of thymus cortex and oxidative damage in aging rats.Methods A total of 40 Wistar rats(weighing 130-170 g)were randomly divided into normal control group,aging group,decoction group and positive control group,with 10 rats in each group.After receiving subcutaneous injection at nape of neck with 1 ml/(kg·d)normal saline in the normal control group and 48 mg/(kg·d)D-galactose in the other 3 groups,the rats of above groups were gavaged distilled water at 10 ml/(kg·d)in the former 2 groups,20 g/(kg·d)decoction in the decoction group,and 0.3 g/(kg·d)vitamin E solution in the positive control group.At the end of 40 days′intervention,all rats were sacrificed,and the contents of total antioxidation capacity(T-AOC),lipid peroxide(LPO)and lactate dehydrogenase(LDH)in the serum were detected.The ultrastructure of thymus cortex was observed with transmission electron microscopy.SPSS statistics 20.0 was used to perform the statistical analysis.One-way analysis of variance was applied for multi-group comparison,and Bonferroni test for comparison between groups.P<0.05 was regarded as statistical significance.Results The body mass of rats in each group was increased significantly on the 14th,28th and 41st days,and the growth rate was slightly lower in the decoction group than the other 3 groups,but there was no significant difference among the groups(all P>0.05).On the 41st day,the T-AOC level was significantly lower while the contents of LDH and LPO were obviously higher in the aging group than the control group(P<0.01).The T-AOC level was remarkably increased while the content of LDH was notably decreased in the decoction group and positive control group than the aging group(P<0.05).The serum LPO content was decreased in the decoction group and the positive control group,but statistical difference was only seen in the former group(P<0.01).The decoction group had higher T-AOC level but lower LPO content when compared with the positive control g

关 键 词：衰老 抗氧化力 胸腺淋巴细胞 胸腺上皮细胞 化痰除湿汤 

分 类 号：R243[医药卫生—中医临床基础] R493[医药卫生—中医学]