双生病毒侵染、烟粉虱取食及其互作对番茄生长及防御基因表达的影响  

Impact of geminivirus infection, whitefly feeding and its interaction on tomato growth and defense gene expression

作　　者：张斐斐[1,2,3,4] 刘圆圆 王炎炎 杨雅云[1,2,3,4] 阿新祥[1,2,3,4] 汤翠凤 董超[1,2,3,4] 王斌 胡剑[1,6] ZHANG Fei-fei;LIU Yuan-yuan;WANG Yan-yan;YANG Ya-yun;A Xin-xiang;TANG Cui-feng;DONG Chao;WANG Bin;HU Jian(Biotechnology and Germplasm Resources Institute,Yunnan Academy of Agriculture Sciences,Kunming 650205,China;Yunnan Provincial Key Lab of Agriculture Biotechnology,Kunming 650205,China;Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation,Kunming 650205,China;Ministry of Agriculture,Scientific Observation for Rice Germplasm Resources of Yunnan,Ministry of Agriculture,Kunming 650205,China;International Agricultural Research Institute,Yunnan Academy of Agriculture Sciences,Kunming 650205,China;Nujiang Green Spice Industry Research Institute,Lushui,Yunnan 673199,China)

机构地区：[1]云南省农业科学院生物技术与种质资源研究所,昆明650205 [2]云南省农业生物技术重点实验室,昆明650205 [3]农业部西南作物基因资源与种质创制重点实验室,昆明650205 [4]农业部云南稻种资源科学观测实验站,昆明650205 [5]云南省农业科学院国际农业研究所,昆明650205 [6]怒江绿色香料产业研究院,云南泸水673199

出　　处：《西南农业学报》2022年第1期113-119,共7页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金(31560500);云南省农业联合专项青年项目[2017FG001-(088)]。

摘　　要：【目的】为深入研究植物病毒—介体昆虫—寄主植物三者互作关系及机制提供科学的理论基础。【方法】以3个不同番茄品种为供试植物,MED烟粉虱为供试昆虫及近年来发现的云南本地双生病毒的优势种PaLCuCNV和TYLCTHV为供试病毒,探究烟粉虱取食、双生病毒侵染及烟粉虱与双生病毒互作对番茄生长发育特性及SA和JA信号途径防御基因表达的影响。【结果】与对照相比,不同处理均能引起番茄植株高度、地上部鲜重和干重的降低,不同处理与不同材料之间差异较大,总体表现为烟粉虱与双生病毒互作较烟粉虱取食、双生病毒侵染对植株高度、地上部鲜重和干重的影响较大。此外,不同处理普遍引起番茄JA和SA信号途径防御基因不同程度的上调表达,只是SA信号途径基因上调幅度较JA信号途径基因的上调幅度大。【结论】植物病毒—介体昆虫—寄主植物之间的互作关系及机制复杂多样,因植物种类、昆虫种类及病毒种类的不同而不同程度地影响植物生长发育及防御基因的表达。【Objective】This study aimed to provide scientific theoretical foundation for further resolving the virus-insect-plant triadic interaction and the underlying mechanism.【Method】Three tomato varieties, MED whitefly, PaLCuCNV and TYLCTHV,the newly-discovered dominant geminivirus in Yunnan province were used to study the impact of whitefly feeding, virus infection and virus-whitefly interaction on the growth, development and the expression of genes in SA and JA signaling pathway.【Result】The results showed that various treatments induced the reduction of plant height, shoot fresh and dry weight of tomatoes, with significant difference between treatments and tomato varieties.It showed a general trend that virus-whitefly interaction had a bigger impact on plant growth and development than just whitefly feeding or virus infection.In addition, defensive genes in SA and JA signaling pathway were up-regulated with varying degree by different treatments, and the degree was higher in SA signaling pathway.【Conclusion】The virus-insect-plant triadic relation and the underlying mechanism was diverse and complicated.Different combination of plant, virus and insects affected the growth and development of tomatoes and the expression of defense response genes in different degree.

关 键 词：烟粉虱 PaLCuCNV TYLCTHV 生长 防御反应 

分 类 号：S641[农业科学—蔬菜学]