草莓杂交品种及其亲本遗传背景的ISSR分析  

Genetic background analysis of strawberry hybrids and parents by ISSR molecular markers

作　　者：张运峰[1,3] 武秋颖 张淑红[1,3] 高凤菊 范永山[1,3] 韩靖玲 刘海英[2] ZHANG Yun-feng;WU Qiu-ying;ZHANG Shu-hong;GAO Feng-ju;FAN Yong-shan;HAN Jing-ling;LIU Hai-ying(Department of Life Sciences,Tangshan Normal University,Tangshan,Hebei 063000,China;Tangshan Academy of Agricultural Sciences,Tangshan,Hebei 063000,China;Tangshan Key Laboratory of Agricultural Pathogenic Fungi and Toxins,Tangshan,Hebei 063000,China)

机构地区：[1]唐山师范学院生命科学系,河北唐山063000 [2]唐山市农业科学研究院,河北唐山063000 [3]唐山市植物病原真菌与毒素重点实验室,河北唐山063000

出　　处：《西南农业学报》2022年第1期162-167,共6页Southwest China Journal of Agricultural Sciences

基　　金：唐山市科技计划项目(19150216E,13120202b)。

摘　　要：【目的】明确草莓杂交品种及其亲本遗传背景。【方法】试验筛选出10条可用于草莓杂交品种及其亲本遗传背景分析的ISSR引物,利用该组引物对12份遗传背景复杂的草莓试验材料进行遗传背景分析。【结果】亲本之间、亲本与子代之间及亲本与组培变异株之间平均遗传距离分别为0.3258、0.2607和0.1981,平均遗传相似系数分别为0.6551、0.7478和0.7672,聚类分析和多维尺度分析均表明遗传背景比较狭窄,并证明本试验中的10条ISSR引物可成为草莓遗传背景分析的有力工具。【结论】草莓遗传背景分析将为其种质创新奠定基础。【Objective】The present paper aimed to clarify the genetic background of strawberry hybrids and their parents.【Method】10 ISSR primers were selected for genetic background analysis of strawberry hybrids and parents.We used the 10 ISSR primers to analyze 12 strawberry cultivars with complex genetic background.【Result】Between the parents, parents and their hybrids, parent and its tissue cultivating variants, the average genetic distances were 0.3258,0.2607 and 0.1981 respectively, and the average genetic similarity coefficient were 0.6551,0.7478 and 0.7672,respectively.The clustering analysis and multidimensional scaling analysis confirmed that strawberry cultivars had a relatively narrow genetic background between the tested materials, indicating that the selected 10 ISSR primers can be a powerful tool for strawberry genetic background analysis.【Conclusion】The genetic background analysis of strawberry will lay a foundation for germplasm innovation.

关 键 词：草莓 遗传背景 ISSR 

分 类 号：S603.6[农业科学—园艺学]