磺胺间甲氧嘧啶对蛋鸡粪便菌群结构的影响  

Effects of sulfamonomethoxine on bacterial community structure in feces of laying hens

作　　者：王静鸽 吉小凤[2,3] 吴静[2,3] 杨华[2,3] 唐标[2,3] 丁保安[1] WANG Jingge;JI Xiaofeng;WU Jing;YANG Hua;TANG Biao;DING Bao an(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China;Institute of Agro-Product Safety and Nutrition,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]青海大学农牧学院,青海西宁810016 [2]浙江省农业科学院农产品质量安全与营养研究所,浙江杭州310021 [3]浙江省农业科学院农产品质量安全危害因子与风险防控国家重点实验室,浙江杭州310021

出　　处：《浙江农业学报》2022年第2期284-292,共9页Acta Agriculturae Zhejiangensis

基　　金：浙江省重点研发计划(2020C02031);国家自然科学基金(31700007);省部共建农产品质量安全危害因子与风险防控国家重点实验室项目(2010DS700124-ZZ2008);浙江省农业重大技术协同推广计划(2021XTTGXM03-3)。

摘　　要：为研究蛋鸡养殖过程中,向饮用水中添加磺胺间甲氧嘧啶(SMM)对蛋鸡粪便菌群结构的影响,选取海兰褐蛋鸡260只,随机平均分为两组(A1组,饮用水中不添加SMM;B1组,饮用水中添加SMM),饲养5 d后采集新鲜粪便,之后B1组停止添加SMM作为B2组,A1组重新编号为A2组,作为B2组的对照组,继续饲养7 d后采集新鲜粪便样品。样品抽提总DNA后,利用Illumina MiSeq高通量测序技术,基于16S rDNA的V3~V4可变区进行多样性分析和粪便细菌群落结构特征分析。结果显示,SMM的使用显著降低了蛋鸡粪便菌群的多样性和丰富度,但乳杆菌属(Lactobacillus)和不动杆菌属(Acinetobacter)的丰度显著(P<0.05)增加。停止使用SMM一周后,蛋鸡粪便菌群的多样性和丰富度得到恢复,不动杆菌属(Acinetobacter)的丰度显著(P<0.05)下降。综上,饲喂SMM改变了蛋鸡肠道的菌群结构,停药后,蛋鸡肠道的菌群可得到恢复。研究结果可为明确抗生素的危害、规范SMM使用等提供参考。In order to explore the effects of sulfamonomethoxine(SMM)addition into the drinking water during breeding on the structure of fecal bacterial community of laying hens,a total of 260 Hailan brown laying hens were randomly divided into two groups(group A1:no SMM was added into drinking water;group B1:SMM was added into drinking water),and fresh feces were collected on the 5th day of feeding.Then,no SMM was added in group B1,and this group was renamed as group B2,and group A1 was renamed as A2(as the control of group B2),and fresh feces were collected again after 7 days of feeding.The total DNA was extracted and sequenced by Illumina MiSeq platform.Based on the variable region of V3-V4 of 16S rDNA,diversity and fecal microbial community structure were analyzed.The addition of SMM reduced the bacterial diversity and richness of the laying hens,but the abundance of Lactobacillus and Acinetobacter were significantly(P<0.05)increased.One week after stopping SMM addtiion,the bacterial diversity and richness of the laying hens recovered,and the abundance of Acinetobacter was significantly(P<0.05)decreased.It was concluded that feeding SMM could change the structure of the intestinal community of laying hens,yet the intestinal community could recover after discontinuation of SMM feeding.The results increased insight into the harm of antibiotics and provided references for the standard use of antibiotics.

关 键 词：磺胺间甲氧嘧啶 蛋鸡 细菌群落结构 

分 类 号：S811.6[农业科学—畜牧学]