黄芩苷-小檗碱复合物纳米晶的制备和在体肠吸收评价  

Preparation and in vivo intestinal absorption evaluation of baicalin-berberine complex nanocrystals

作　　者：刘羿廷 马旭彤 王继林 彭辉 皮佳鑫[1,2] 刘志东[1,2] LIU Yiting;MA Xutong;WANG Jilin;PENG Hui;PI Jiaxin;LIU Zhidong(State Key Laboratory of Component Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique,Ministry of Education,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]天津中医药大学组分中药国家重点实验室,天津301617 [2]天津中医药大学现代中药发现与制剂技术教育部工程中心,天津301617

出　　处：《天津中医药大学学报》2022年第1期102-107,共6页Journal of Tianjin University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81803739)。

摘　　要：[目的]研究纳米晶对黄芩苷-小檗碱复合物的肠吸收促进作用。[方法]使用抗溶剂法结合高压均质法制备黄芩苷-小檗碱复合物纳米晶,进行初步体外评价。建立在体单向肠灌流模型,评价纳米晶对复合物吸收的促进效果。[结果]制备了黄芩苷-小檗碱复合物纳米晶。体外评价结果表明,黄芩苷-小檗碱复合物纳米晶为圆柱状,粒径为(225.10±9.12)nm,多分散系数为(0.27±0.04)。在十二指肠、空肠、回肠、结肠4个肠段,纳米晶组黄芩苷的有效渗透系数(P_(eff))分别是复合物组的2.99、2.00、2.66、5.58倍,纳米晶组小檗碱的P_(eff)分别是复合物组的1.25、1.18、1.53、1.80倍。[结论]纳米晶提高了黄芩苷-小檗碱复合物的在体肠吸收。[Objective]To investigate the intestinal permeability improvement of baicalin-berberine complex nanocrystals.[Methods]Baicalin-berberine complex nanocrystals were prepared by anti-solvent methodfollowed by high pressure homogenization,and the in situ single-pass intestinal perfusion model was established to evaluate the absorption enhancement of the complex nanocrystals.[Results]Baicalin-berberine complex nanocrystals were prepared and characterized.The baicalin-berberine complex nanocrystals were cylindrical with the particle size at(225.10±9.12)nm,and the Polymer dispersity index was(0.27±0.04).In duodenum,jejunum,ileum and colon,the effective permeability coefficients(P_(eff))of baicalin in nanocrystals group were 2.99,2.00,2.66 and 5.58 times higher than that of coarse suspension group,separately.The P_(eff) of berberine in nanocrystals group were 1.25,1.18,1.53 and 1.80 times higher than that of coarse suspension group,respectively.[Conclusion]Nanocrystals could improve the oral absorption of baicalin-berberine complex.

关 键 词：中药 纳米晶 黄芩苷 小檗碱 在体单向肠灌流模型 吸收 

分 类 号：R943[医药卫生—药剂学]