茎瘤芥异戊烯基转移酶家族基因全基因组鉴定及表达分析  

Genome-wide Identification and Expression Analysis of Isopentenyl Transferases Genes in Tuber Mustard

作　　者：蔡兆明[1] 程春红[1] 廖静静 王殿东[1] CAI Zhaoming;CHENG Chunhong;LIAO Jingjing;WANG Diandong(College of Life Science and Technology, Yangtze Normal University, Chongqing 408100, China)

机构地区：[1]长江师范学院生命科学与技术学院,重庆408100

出　　处：《西北植物学报》2022年第1期38-47,共10页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31800311);重庆市科技局项目(cstc2019jcyj-msxmX0285);重庆市教育委员会项目(KJQN201901406);长江师范学院青年人才(2018QNRC20)。

摘　　要：该研究以茎瘤芥栽培品种‘永安小叶’为实验材料,在全基因组水平对茎瘤芥基因组中异戊烯基转移酶(IPT)家族基因成员进行鉴定;通过荧光定量PCR检测各基因在不同组织、盐胁迫和根肿菌胁迫条件下的表达模式。结果显示:(1)在茎瘤芥基因组中共鉴定到27个IPT家族基因,分布在14条染色体上,它们在系统进化树中可聚类为7个分支。(2)大部分IPT家族基因主要在茎瘤芥的根和茎中表达,在叶片、花和种荚中表达量相对较低。BjuB006281在茎中的表达水平最高,BjuA027211、BjuB010173、BjuB010174和BjuA001839在根中的表达水平较高。(3)大部分的IPT基因表达受盐胁迫抑制,BjuB006281、BjuA036403、BjuB010173、BjuB026254在盐胁迫12~48 h显著下调表达;BjuB022918和BjuB007352则在盐胁迫24~48 h显著下调表达。(4)大部分茎瘤芥IPT基因在12 h受到根肿菌侵染的显著诱导,其中BjuB006281、BjuA014415、BjuB022918在侵染后12 h的表达水平为0 h对照的15倍以上。该研究鉴定出多个响应盐胁迫和根肿菌胁迫的IPT基因,为进一步研究他们的基因功能奠定了基础。In this study,the tuber mustard cultivar‘Yongan Xiaoye’was selected as experimental material,and the isopentenyl transferases(IPT)family genes were identified in its genome.The fluorescent quantitative PCR were used to test the IPT gene expression patterns in different organs and under salt and Plasmodiophora brassicae treatments.The results showed:(1)twenty-seven IPT genes were identified in the genome of tuber mustard,which were located on 14 of 18 chromosomes,the IPT proteins attributed to 7 clades in the phylogenetic tree.(2)The majority of IPT genes were highly expressed in roots and stems,little genes were expressed in leaves,flowers and pods.BjuB006281 were highly expressed in stems,and BjuA027211,BjuB010173,BjuB010174 and BjuA001839 were expressed higher in roots than the other organs.(3)Most of the IPT genes were down-regulated by salt stress treatment,the expression of BjuB006281,BjuA036403,BjuB010173 and BjuB026254 were reduced between 12-48 h after salt stress treatment,while BjuB022918 and BjuB007352 were reduced between 24-48 h.(4)The majority of IPT genes were induced by P.brassicae treatment at 12 h,for example,the expression levels of BjuB006281,BjuA014415 and BjuB022918 were increased more than 15 folds comparing to the 0 h control.In together,several salt and P.brassicae responsive genes were identified in this study,which could provide a basis for further studying their functions.

关 键 词：茎瘤芥 异戊烯基转移酶 盐胁迫 根肿菌 基因家族 

分 类 号：Q786[生物学—分子生物学] Q789