miR-652对非小细胞肺癌H460细胞凋亡和迁移的影响  

Effects of miR-652 on Apoptosis and Migration of Non-small Cell Lung Cancer H460 Cells

作　　者：王建宇 马文俊 王莹[1] WANG Jian-Yu;MA Wen-Jun;WANG Ying(Basic Medical College,Mudanjiang Medical College of Mudanjiang Medical University,Mudanjiang 157011,China)

机构地区：[1]牡丹江医学院基础医学院,黑龙江牡丹江157011

出　　处：《中国药物经济学》2022年第1期44-46,55,共4页China Journal of Pharmaceutical Economics

基　　金：黑龙江省省属高等学校基本科研业务费科研项目(2017-kyywfmy-0679)。

摘　　要：目的观察miR-652对非小细胞肺癌H460细胞凋亡和迁移的影响。方法应用细胞计数实验检测miR-652的浓度对肺癌H460细胞的影响;应用细胞划痕实验检测miR-652对肺癌H460细胞迁移的影响;应用蛋白质免疫印迹技术检测肺癌H460细胞凋亡蛋白表达情况。结果在H460细胞中,miR-652表达显著减少;转染miR-652后,H460细胞计数减少,细胞迁移速度下降,同时细胞内Bax和caspase-9蛋白表达显著增加。结论miR-652的过表达抑制H460肺癌细胞的迁移,并促进其凋亡。Objective To observe the effects of miR-652 on apoptosis and migration of non-small cell lung cancer(NSCLC)H460 cells.Methods The cell counting experiment was used to detect the effect of miR-652 concentration on lung cancer H460 cells;the cell scratch test was used to detect the effect of miR-652 on lung cancer H460 cell migration;the western blot technique was used to detect the expression of apoptotic protein in lung cancer H460 cells.Results In H460 cells,the expression of miR-652 was significantly reduced;after miR-652 was transfected,the cell count of H460 decreased,the cell migration rate decreased,and the expression of Bax and caspase-9 proteins in the cells increased significantly.Conclusion Overexpression of miR-652 inhibits the migration of H460 lung cancer cells and promotes their apoptosis.

关 键 词：非小细胞肺癌 miR-652 H460细胞 BAX 迁移 凋亡 

分 类 号：R374[医药卫生—病原生物学]