3D细胞模型在化学物质毒性测试中的应用研究进展  

Progress in the application of three-dimensional cell culture model in toxicity tests of xenobiotic

作　　者：王紫微 张睿 陈雯[1] Wang Ziwei;Zhang Rui;Chen Wen(Department of Toxicology,School of Public Health,Sun Yat-sen University,Guangzhou 510080,China)

机构地区：[1]中山大学公共卫生学院卫生毒理学系,广州510080

出　　处：《中华预防医学杂志》2022年第1期20-24,共5页Chinese Journal of Preventive Medicine

基　　金：国家重点研发计划(2017YFC1600200)。

摘　　要：在化学物质毒性预测及风险评估中,体外细胞模型具有较高的实际应用价值。随着3D生物打印、类器官培养、器官芯片等技术的迅速发展,体外细胞模型取得了长足的进步。相比起2D细胞模型,3D细胞模型在结构、功能及生理环境上与在体组织、器官更为接近。基于3D细胞模型进行化学物质的危害识别及剂量-反应关系分析,可以获得较为准确的毒性数据,为化学物质风险评估及风险管理提供理论依据。本文概述了三种典型的3D细胞模型(人源细胞系共培养模型、3D生物打印细胞模型和类器官模型)的构建及其在化学物质毒性测试中的应用前景。In the process of xenobiotic toxicity prediction and risk assessment,in vitro cell culture models possess high practical application value.With the rapid development of biological technologies such as three-dimensional(3D)bio-printing,organoid culture and organ-on-a-chip systems,in vitro cell culture models have made great progress.Sharing the similarities in structure,function and the physiological environment with tissues or organs in vivo,hazard identification and dose-response analysis based on 3D cell culture models provide access to more accurate toxicity data as a theoretical basis for risk assessment and risk management of chemicals.This review summarizes the establishment of three typical 3D cell culture models,i.e.,human cell line-based co-culture model,3D-printed scaffold-based cell culture model and organoids,and their application in toxicity tests of xenobiotics.

关 键 词：毒性试验 危害物质 3D细胞模型 外源性化学物 

分 类 号：R99[医药卫生—毒理学]