子宫内膜异位症患者血清中PAPP-A,IGF-I水平及PAPP-A基因rs7020782 A/C位点多态性与临床分期相关性研究  

Study on the Correlation between the Levels of PAPP-A,IGF-I and PAPP-A Gene rs7020782 A/C Loci Polymorphism and Clinical Stage in Patients with Endometriosis

作　　者：曾辉苑 张燕 刘国栋 林丽 刘爱胜 梁秀文 ZENG Hui-yuan;ZHANG Yan;LIU Guo-dong;LIN Li;LIU Ai-sheng;LIANG Xiu-wen(Department of Obstetrics and Gynecology,Shenzhen Longhua District People’s Hospital,Guangdong Shenzhen 518109,China;Department of Clinical Laboratory,Shenzhen Longhua District People’s Hospital,Guangdong Shenzhen 518109,China)

机构地区：[1]深圳市龙华区人民医院妇产科,广东深圳518109 [2]深圳市龙华区人民医院检验科,广东深圳518109

出　　处：《现代检验医学杂志》2022年第1期33-37,共5页Journal of Modern Laboratory Medicine

基　　金：深圳市龙华区科技创新局科研项目(编号:2021092)。

摘　　要：目的研究子宫内膜异位症(endometriosis,EMs)患者血清中妊娠相关血浆蛋白-A(pregnancy-associated plasma protein-A,PAPP-A)、胰岛素样生长因子-I(insulin-like growth factor-I,IGF-I)水平及PAPP-A基因rs7020782 A/C位点单核苷酸多态性(SNP)与临床分期的相关性。方法选择EMs确诊者176例作为EMs组,同期非EMs育龄妇女120例作为对照组。分别检测血清中PAPP-A和IGF-I水平,同时分析PAPP-A基因rs7020782 A/C位点SNP。结果EMs组PAPP-A和IGF-I水平(16.38±5.29mIU/L和145.47±18.02ng/ml)明显高于对照组(7.42±2.83mIU/L和81.34±12.61ng/ml),差异均有统计学意义(t=7.0185,5.6241,均P<0.05)。PAPP-A和IGF-I水平随临床分期增加而升高,不同分期之间差异均有统计学意义(F=14.6083,12.3715,均P<0.05)。PAPP-A与IGF-I水平呈正相关(r=0.7902,P=0.0261),且PAPP-A和IGF-I水平与临床分期呈正相关(r=0.6127,0.7358,均P<0.05)。EMs组PAPP-A基因rs7020782 A/C位点CC基因型和C等位基因频率(68.75%和78.41%)明显高于对照组(46.67%和55.83%),差异有统计学意义(χ^(2)=6.0523,4.3921,均P<0.05),且CC基因型和C等位基因患EMs的相对风险增加(OR=3.095,95%CI:1.982~6.873和OR=2.607,95%CI:1.821~4.759)。携带CC基因型EMs患者PAPP-A水平(18.48±7.13mIU/L)明显高于AC和AA基因型(12.03±3.86mIU/L和11.42±3.51mIU/L),差异均有统计学意义(t=6.8412,7.0614,均P<0.05),但AC和AA基因型差异无统计学意义(t=0.8524,P>0.05)。结论EMs患者血清中PAPP-A和IGF-I水平明显升高,且与临床分期有关,可能与EMs发生有密切关系。同时PAPP-A基因rs7020782 A/C位点呈多态性,其中CC基因型可能与该地区EMs患病具有一定的相关性。Objective To Study on the correlation between the levels of pregnancy-associated plasma protein-A(PAPPA),insulin-like growth factor-I(IGF-I)and PAPP-A gene rs7020782 A/C loci polymorphism and clinical stage in patients with endometriosis(EMs).Methods 176 patients diagnosed with EMS were selected as the EMS group,and 120 non-EMS women of childbearing age were selected as the control group.The levels of PAPP-A and IGF-I were detected,and the SNP of PAPP-A gene rs7020782 A/C was analyzed.Results The levels of PAPP-A and IGF-I in EMS group(16.38±5.29mIU/L and 145.47±18.02ng/ml)were significantly higher than those in control group(7.42±2.83mIU/L and 81.34±12.61ng/ml),and the difference was statistically significant(t=7.0185,5.6241,all P<0.05).The levels of PAPP-A and IGF-I increased with the increase of clinical stage,and the differences among different stages were statistically significant(F=14.6083,12.3715,all P<0.05).There was positive correlation between PAPP-A and IGF-I level(r=0.7902,P=0.0261),and there were positive correlation between the levels of PAPP-A and IGF-I and clinical stage(r=0.6127,0.7358,all P<0.05).CC genotype and C allele frequency of PAPP-A rs7020782A/C locus in EMS group(68.75%and 78.41%)were significantly higher than those in control group(46.67%and 55.83%),the difference were statistically significant(χ^(2)=6.0523,4.3921,all P<0.05).CC genotype and C allele were associated with an increased relative risk of EMS(OR=3.095,95%CI:1.982~6.873 and OR=2.607,95%CI:1.821~4.759).The level of PAPP-A in EMS patients with CC genotype(18.48±7.13 mIU/L)was significantly higher than that of AC and AA genotypes(12.03±3.86 mIU/L and 11.42±3.51 mIU/L),and the difference were statistically significant(t=6.8412,7.0614,all P<0.05),but there was no statistically significant difference between AC and AA genotypes(t=0.8524,P>0.05).Conclusion The serum levels of PAPP-A and IGF-I in EMS patients were significantly increased,which was related to the clinical stage,and may be closely related to the occurrence of

关 键 词：子宫内膜异位症 妊娠相关血浆蛋白-A 胰岛素样生长因子-I 基因 单核苷酸多态性 

分 类 号：R711.71[医药卫生—妇产科学] R392.11[医药卫生—临床医学]