南极海绵共附生真菌Talaromyces sp.HDN1820200次级代谢产物研究  

Study on the secondary metabolites of an Antarctica spongederived fungus Talaromyces sp.HDN1820200

作　　者：赵漪 孙春晓 侯朝霞 李培霞 车茜[1] 张国建[1] 李德海[1] 朱天骄[1] ZHAO Yi;SUN Chun-xiao;HOU Zhao-xia;LI Pei-xia;CHE Qian;ZHANG Guo-jian;LI De-hai;ZHU Tian-jiao(Key Laboratory of Marine Drugs,Ministry of Education,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China)

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,医药学院,山东青岛266003

出　　处：《中国海洋药物》2022年第1期24-30,共7页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(41876216);国家重点研发计划项目(2018YFC1406705);新药创制国家科技重大专项(2018ZX09735004)资助。

摘　　要：目的对南极海绵共附生来源真菌Talaromyces sp.HDN1820200的次级代谢产物进行研究。方法对菌株发酵液粗提物利用柱层析(VLC,ODS)、凝胶分子筛(LH-20)以及半制备高效液相(MPLC)等方法进行次级代谢产物的分离纯化;利用现代波谱学方法(核磁共振NMR、圆二色谱ECD、红外光谱IR等)对化合物进行结构鉴定。结果分离鉴定了8个单体化合物:(2E,4E,6E)-4-methyldodeca-2,4,6-trienoic acid(1)、(2E,4Z,6E)-4-methyldodeca-2,4,6-trienoic acid(2)、Herbaridine B(3)、Kasanosin C(4)、Citrinolactone A(5)、Citreorosein(6)、1,5,8-trihydroxy-3-(hydroxymethyl)anthracene-9,10-dione(7)和Rugulosin B(8)。结论从南极海绵来源真菌Talaromyces sp.HDN1820200中分离得到了化合物1~8,其中化合物1和2为新化合物,属于脂肪酸类化合物,无明显细胞毒活性。Objective To study the secondary metabolites of an Antarctica sponge-derived fungus Talaromyces sp.HDN1820200.Methods The crude extract was separated and purified by column chromatography(VLC,ODS),sephadex LH-20 and medium pressure preparative liquid chromatography(MPLC),etc.The structures of compounds were elucidated with the modern spectroscopy methods(NMR,CD,IR,etc.)as well as the related literature.Results Eight compounds were obtained and identified as(2E,4E,6E)-4-methyldodeca2,4,6-trienoic acid(1),(2E,4Z,6E)-4-methyldodeca-2,4,6-trienoic acid(2),Herbaridine B(3),Kasanosin C(4),Citrinolactone A(5),Citreorosein(6),1,5,8-trihydroxy-3-(hydroxymethyl)anthracene-9,10-dione(7)and Rugulosin B(8).Conclusion Eight compounds(1−8)were isolated from the sponge-derived fungus Talaromyces sp.HDN1820200.Among them,1 and 2 were new compounds,which belonged to fatty acid compounds with no obvious cytotoxicity activity.

关 键 词：南极 海绵共附生微生物 Talaromyces sp. 次级代谢产物 脂肪酸 

分 类 号：R931.77[医药卫生—生药学]