1株海洋来源真菌Aspergillus alabamensis次级代谢产物研究  

Study on the secondary metabolites of a marine fungus Aspergillus alabamensis

作　　者：孙彩霞 唐旭利[2] 李凯 李国强[1] 李平林[1] SUN Cai-xia;TANG Xu-li;LI Kai;LI Guo-qiang;LI Ping-lin(Key Laboratory of Marine Drugs,Ministry of Education,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China;College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266100,China)

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,医药学院,山东青岛266003 [2]中国海洋大学化学化工学院,山东青岛266100

出　　处：《中国海洋药物》2022年第1期63-69,共7页Chinese Journal of Marine Drugs

基　　金：山东省重大科技创新工程专项(2018SDKJ0401-3)资助。

摘　　要：目的对来源于西沙群岛的海绵(Agelas sp.)共附生微生物Aspergillus alabamensis(18-xs-01-zp-3)进行菌种鉴定及次级代谢产物的研究。方法通过分子生物学方法鉴定菌株种属;综合运用硅胶柱层析、薄层色谱和半制备高效液相色谱(HPLC)等色谱学方法对次级代谢产物进行分离纯化,通过核磁共振(NMR)、质谱(MS)等现代波谱学方法并结合文献对所得化合物进行结构鉴定。结果从中分离得到7个单体化合物,分别是ardeemin型生物碱3个:5-N-acetyl-15bβ-hydroxyardeemin(1)、5-N-acetylardeemin(2)、5-N-acetyl-15b-didehydroardeemin(3);二酮哌嗪生物碱2个:3-benzylidene-3,4-dihydro-4-methyl-lH-l,4-benzodiazepine-2,5-dione(4)、benzodiazepinedione(5);吲哚类生物碱2个:luteoride E(6)、14-hydroxyterezine D(7)。结论从该菌株共分离得到7个生物碱类化合物,化合物1对H69AR具有抗肿瘤活性,化合物4为首次从曲霉属真菌中分离得到。Objective To identify the species and study the secondary metabolites of Aspergillus alabamensis(18-xs-01-zp-3),a symbiotic microorganism isolated from the sponge(Agelas sp.)collected from the Paracel Islands.Methods The genus of the strain was identified by molecular biological method.The secondary metabolites were separated and purified by silica gel column chromatography,thin layer chromatography and semi-HPLC column chromatography,and their structures were determined by NMR,MS and other spectral methods,and comparison with previously reported data.Results Seven alkaloids were isolated from the secondary metabolites of A.alabamensis,including three ardeemin alkaloids:5-N-acetyl-15bβhydroxyardeemin(1),5-N-acetylardeemin(2),5-N-acetyl-15b-didehydroardeemin(3),two diketopiperazine alkaloids:3-benzylidene-3,4-dihydro-4-methyl-1H-1,4-benzodiazepine-2,5-dione(4),benzodiazepinedione(5);two indole alkaloids:luteoride E(6),14-hydroxyterezine D(7).Conclusion A total of seven alkaloids were isolated from this strain,compound 1 showed antineoplastic activity on H69AR,compound 4 was isolated from fungus Aspergillus sp.for the first time.

关 键 词：共附生微生物 Aspergillus alabamensis 生物碱 

分 类 号：R931[医药卫生—生药学]