华蟾素抑制人鼻咽癌细胞系CNE2和HONE1增殖  

Cinobufagin inhibits proliferation of human nasopharyngeal carcinoma cell lines CNE2 and HONE1

作　　者：赵霞[1] 蔡庆春[1] 丁瑞敏[1] 张倩[1] ZHAO Xia;CAI Qing-chun;DING Rui-min;ZHANG Qian(The Third Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450004, China)

机构地区：[1]河南中医药大学第三附属医院,河南郑州450004

出　　处：《基础医学与临床》2022年第3期436-440,共5页Basic and Clinical Medicine

基　　金：河南省中医管理局基金(2018ZY1013)。

摘　　要：目的评估华蟾素(Cin)对人鼻咽癌(NPC)细胞系(CNE2和HONE1)的生物学作用以及发挥功效的机制。方法用不同浓度梯度(0、5、15和45 mg/L)Cin分别处理CNE2和HONE1细胞24、48和72 h后,用CCK-8法检测细胞活性。48 h后,用相差显微镜观察细胞形态;TUNEL荧光染色法检测细胞凋亡;DCF荧光法评估细胞内的活性氧簇(ROS)水平;蛋白质免疫印迹法检测凋亡相关蛋白表达。结果Cin呈浓度依赖性抑制NPC细胞活性(P<0.05,P<0.01和P<0.001),并且导致细胞出现拉丝和稀疏现象。Cin促进NPC细胞凋亡并增加细胞内ROS水平(P<0.05,P<0.01或P<0.001)。Cin抑制Bax和线粒体细胞色素c(Cytc)的表达(P<0.05,P<0.01或P<0.001),但促进Bcl-2、cleaved caspase-3和胞质Cytc的表达(P<0.01或P<0.001)。结论Cin可抑制NPC细胞的增殖并促进细胞凋亡。Objective To evaluate the biological effect of cinobufagin(Cin)on nasopharyngeal carcinoma(NPC)cells lines(CNE2 and HONE1)and the mechanism of its efficacy.Methods CNE2 and HONE1 cells were treated with different concentrations(0,5,15 and 45 mg/L)of Cin for 24,48 and 72 h respectively and cell viability was detected by CCK-8 assay.After 48 hrs,cell morphology was observed by phase contrast microscopy;cell apoptosis was detected by TUNEL fluorescence staining;the level of reactive oxygen species(ROS)in cells were assessed by DCF fluorescence assay;the expression levels of apoptosis-related proteins were detected by Western blot.ResultsCin inhibited the cell activity of NPC cells in a concentration-dependent manner(P<0.05,P<0.01 or P<0.001)and the cells showed filament and sparse morphology.Cin promoted apoptosis and increased intracellular ROS level in NPC cells(P<0.05,P<0.01 or P<0.001).Cin inhibited the expression of Bax and mitochondrial cytochrome c(Cytc)(P<0.05,P<0.01 or P<0.001)but promoted the expression of Bcl-2,cleaved caspase-3 and cytoplasmic Cytc(P<0.01 or P<0.001).Conclusions Cin can inhibit cell proliferation and promote apoptosis of NPC cells.

关 键 词：华蟾素 鼻咽癌 凋亡 增殖 

分 类 号：R739.62[医药卫生—肿瘤]