激光扫描共聚焦显微镜活细胞成像方法优化  

Optimization of Live Cell Imaging with Laser Scanning Confocal Microscopy

作　　者：张彦丽 刘冰钰 陈亚兰 王瑾瑜 曹慧珍[1,3] ZHANG Yan-li;LIU Bing-yu;CHEN Ya-lan;WANG Jin-yu;CAO Hui-zhen(School of Life Sciences,Tsinghua University,Beijing,100084,China;Technology Center For Protein Sciences,Tsinghua University,Beijing,100084,China;Center of Beijing Biomedical Analysis,Tsinghua University,Beijing,100084,China)

机构地区：[1]清华大学生命科学学院,北京100084 [2]清华大学蛋白质研究技术中心,北京100084 [3]清华大学生物医学测试中心,北京100084

出　　处：《现代生物医学进展》2022年第1期196-200,共5页Progress in Modern Biomedicine

基　　金：清华大学实验室创新基金(53100100519)。

摘　　要：激光扫描共聚焦显微镜可用于固定样品和活细胞样品的成像,近年来得到了广泛的应用。本文介绍了激光扫描共聚焦显微镜的基本原理及其在活细胞成像中的应用,并以FV10-ASW Viewer4.2软件为例,从扫描速度、分辨率、降噪、光电倍增调节、多参数协同优化、成像质量评估、图像后期处理等多个角度总结了激光扫描共聚焦活细胞成像系统的方法优化和推荐参数设置。本文的工作可以为活细胞实验提供一定参考。The laser scanning confocal microscope(LSCM) has been widely used in the imaging of fixed and live cell samples.We introduced the basic principle of LSCM and its application in live cell imaging. Take FV10-ASW Viewer4.2 software as an example,we summarized the optimization and recommended parameter settings of live cell imaging system from the aspect of scanning speed,resolution, noise reduction, photomultiplier, multi-parameter collaborative optimization, imaging quality evaluation and post-processing.This work may provide some reference for live cell experiment.

关 键 词：激光扫描共聚焦显微镜 活细胞成像 参数优化 

分 类 号：TN16[电子电信—物理电子学] O657.3[理学—分析化学]