Recombinant Pyriform Spider Silk Expression and Wet-Spinning  

作　　者：LIEBIEDIEV Pavlo LIN Ying LIEBIEDIEV Pavlo;林瑛(College of Chemistry,Chemical Engineering and Biotechnology,Donghua University,Shanghai 201620,China)

机构地区：[1]College of Chemistry,Chemical Engineering and Biotechnology,Donghua University,Shanghai 201620,China

出　　处：《Journal of Donghua University(English Edition)》2022年第1期28-34,共7页东华大学学报（英文版）

基　　金：National Natural Science Foundation of China(No.31470836);Science and Technology Commission of Shanghai Municipality,China(No.19ZR1471000)。

摘　　要：Spider silk is capturing the attention of scientists for its mechanical properties,biocompatibility,and biodegradability.Spiders can produce six types of silks,as well as special glue,which are used for survival and reproduction.During the last years of research,scientists deciphered gene sequences and expressed the most common types of spider silks.However,matching the mechanical properties of recombinant spider silks to native ones is still a big challenge.Moreover,in-depth studies are mostly focused on natural and recombinant ampullate silks,and only a few studies showed achievements on pyriform spidroin(PySp).In this study,repeatable parts of PySp were expressed,purified,and spun into fibers.Recombinase cloning strategy allowed to create highly-repetitive region parts clones efficiently in comparison to the traditional restriction enzyme cloning technique.A cost-effective high-yield purification strategy was used.This study provides strategies that can help to design recombinant spider silks with the same mechanical properties as native spider silks.

关 键 词：spidroin expression protein purification pyriform spidroin(PySp) recombinant spider silk WET-SPINNING mechanical property 

分 类 号：Q816[生物学—生物工程]