利用微卫星标记分析湖羊群体遗传多样性  

Genetic diversity assessment of Hu sheep population using microsatellite DNA markers

作　　者：李丹阳 吴彩凤[1] 孙玲伟 张树山[1] 张德福[1] 戴建军[1] LI Danyang;WU Caifeng;SUN Lingwei;ZHANG Shushan;ZHANG Defu;DAI Jianjun(Institute of Animal Husbandry and Veterinary Science,Shanghai Academy of Agricultural Sciences,Division of Animal Genetic Engineering Shanghai Municipal Key Laboratory of Agri-genetics and Breeding,Shanghai 201106,China;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海市农业遗传育种重点实验室动物遗传工程研究室,上海201106 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《上海农业学报》2022年第1期67-72,共6页Acta Agriculturae Shanghai

基　　金：上海市科技兴农项目[沪农科创字(2020)第1-5号];上海市科委实验动物专项(19140900100)。

摘　　要：利用微卫星标记技术分析湖羊群体遗传多样性。结果表明:31个湖羊样本共检测出等位基因210个,Shannon指数I为1.416,多态信息含量PIC为0.634,观察杂合度Ho为0.692,期望杂合度He为0.685,Ewens-Watterson检测所有位点都处于置信区间内。公羊Ho为0.694,He为0.643,I为1.190,PIC为0.564;母羊Ho为0.676,He为0.693,I值为1.403,PIC为0.636,表明群体遗传多样性丰富,变异程度高。等位基因频率分布呈L型,各位点均值处于Hard-Weinberg平衡,表明湖羊群体未出现瓶颈效应。研究结果为湖羊育种奠定了一定基础。The genetic diversity of Hu sheep population was analyzed using microsatellite marker technology.The results showed that a total of 210 alleles were detected out from 31 samples of Hu sheep,the Shannon index(I)was 1.416,polymorphic information content(PIC)was 0.634,the observed heterozygosity(Ho)was 0.692,and the expected heterozygosity(He)was 0.685.All the locis detected by Ewens-Watterson were within the confidence interval.According to results of population analysis,Ho,He,I and PIC of ram were 0.694,0.643,1.190 and 0.564,respectively;Ho,He,I and PIC of ewe were 0.676,0.693,1.403 and 0.636,respectively,which indicated that the genetic diversity of Hu sheep varieties was rich.The allele frequency distribution was L-shaped,the mean value of each loci was in Hard-Weinberg balance,which indicated that there was no bottleneck effect in this population.In conclusion,the population of Shanghai Hu sheep had high genetic diversity and no bottleneck effect.The research results laid a foundation for the breeding of Hu sheep.

关 键 词：湖羊群体 微卫星 遗传多样性 瓶颈效应 

分 类 号：S826[农业科学—畜牧学]