双重PCR技术鉴定番茄Sw-5和Ty-2基因  

Identification of Sw-5 and Ty-2 Gene by Using Double PCR Technique

作　　者：王涛[1] 张子君[1] 张逸鸣[1] 王晓峰[1] 邹庆道[1] WANG Tao;ZHANG Zi-jun;ZHANG Yi-ming;WANG Xiao-feng;ZOU Qing-dao(Liaoning Academy of Agricultural Sciences,Institute of Vegetables,Shenyang,Liaoning 110161)

机构地区：[1]辽宁省农业科学院蔬菜研究所,辽宁沈阳110161

出　　处：《辽宁农业科学》2022年第1期35-38,共4页Liaoning Agricultural Sciences

基　　金：辽宁省百千万人才工程项目(XLYC1905009)。

摘　　要：研究利用双重PCR技术同时鉴定番茄抗斑萎病毒病基因Sw-5和抗番茄黄化曲叶病毒病基因Ty-2,鉴定结果与单引物PCR完全吻合。其中Sw-5的连锁标记在纯合抗病和感病材料中分别扩增出574 bp和464 bp的特异条带,在杂合抗病材料中可同时扩增出以上两条带;Ty-2的连锁标记在纯合抗病和感病材料中分别扩增出900 bp和800 bp的特异条带,在杂合抗病材料中同样扩增出两条带。利用双重PCR方法对17份待检测番茄材料进行鉴定,条带清晰,且辨识度高。该技术极大地提高了Sw-5和Ty-2基因的鉴定效率。We identified tomato spotted wilt virus resistance gene Sw-5 and tomato yellow leaf curl virus resistance gene Ty-2 simultaneously by using double PCR Technique. The results were completely in consistent with the amplified bands of regular PCR. Among them, the linked marker of Sw-5 amplified 574 bp and 464 bp bands, respectively, in tomato homozygous resistant and susceptible materials. While in heterozygous resistant lines, it amplified two bands. The linked marker of Ty-2 produced 900 bp and 800 bp bands, respectively, in tomato homozygous resistant and susceptible materials, and two bands were amplified in the heterozygous resistant lines. Genotype identification of 17 tomato materials was carried out using this technology, the amplified bands were clear and highly recognizable. This technique greatly improved the efficiency of identifying Sw-5 and Ty-2 gene.

关 键 词：番茄 双重PCR Sw-5 Ty-2 

分 类 号：Q781[生物学—分子生物学]