毫针针刺对SD大鼠腧穴筋膜组织CSK、ATP和PCNA影响  

The Effect of CSK, ATP and PCNA in the Fascia Tissues of Acupoints on SD Ratsby Filiform Needle Acupuncture

作　　者：胡铁汉 李程 司原成 陈波 张婷 赖清 HU Tie-han;LI Chen;SI Yuan-cheng;CHEN Bo;ZHANG Ting;LAI Ting(College of Acupuncture and Tuina,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,Guizhou,China;Department of Traditional Chinese Medicine,Guizhou Second People's Hospital,Guiyang 550004,Guizhou,China)

机构地区：[1]贵州中医药大学针灸推拿学院,贵州贵阳550025 [2]贵州省第二人民医院中医科,贵州贵阳550004

出　　处：《医学信息》2022年第3期168-171,共4页Journal of Medical Information

基　　金：国家自然科学基金(编号:81860882、81160456);贵州省科技基金(编号:黔科合基础【2018】1018)。

摘　　要：目的研究毫针针刺对腧穴筋膜组织细胞骨架(CSK)的调节作用及其对三磷酸腺苷(ATP)含量、增殖细胞核抗原(PCNA)表达的影响,为针刺作用原理提供新的实验证据。方法将16只SPF级SD大鼠,随机均分为空白组(n=8)和针刺组(n=8),针刺组针刺中脘穴,捻转行针(60±3)次/min,共5 min,留针10 min,共操作3轮后出针,1次/d,连续3 d。术毕剪取以大鼠中脘穴的穴点为圆心、直径约0.5 cm区域的筋膜组织块。采用免疫组织化学法检测CSK微丝(actin)、中间丝(vimentin)、微管(tubulin)和PCNA的差异变化,应用ELISA法检测组织中ATP的含量差异。结果针刺组的CSK微丝、微管表达的平均光密度(Mean density,MD)均低于空白组(P<0.01);两组中间丝的表达比较,差异无统计学意义(P>0.05);针刺组腧穴组织ATP含量及PCNA阳性细胞计数率均高于空白组(P<0.01)。结论毫针针刺可能通过细胞骨架通路,增强腧穴组织能量代谢和细胞活力,进而激发针刺治疗作用,是现代针灸医学研究的机理之一。Objective To study the regulatory effect of filiform needle acupuncture on the cytoskeleton(CSK)of acupoints'fascia tissue and its effect on ATP content and PCNA expression,so as to provide new experimental evidence for the mechanism of acupuncture.Methods A total of 16 SPF SD rats were randomly divided into blank group(n=8)and acupuncture group(n=8).The acupuncture group was treated with acupuncture at Zhongwan acupoint,twisting the needle(60±3)times/min for 5 min,retaining the needle for 10 min,and the needle was removed after 3 rounds of operation,once daily for 3 days.At the end of the operation,the fascia tissue blocks with the central point of Zhongwan point in rats as the center and the diameter of about 0.5 cm were cut.Immunohistochemistry was used to detect the difference of actin,vimentin,tubulin and PCNA in CSK,and ELISA was used to detect the difference of ATP content in tissues.Results The mean density(MD)of CSK filaments and microtubules in acupuncture group was lower than that in blank group(P<0.01);there was no significant difference in the expression of interfilament between the two groups(P>0.05).ATP content and PCNA positive cell count rate in acupuncture group were higher than those in blank group(P<0.01).Conclusion Filiform needle acupuncture may enhance the energy metabolism and cell vitality of acupoints through the cytoskeleton pathway,thereby stimulating the therapeutic effect of acupuncture,which is one of the mechanisms of modern acupuncture medical research.

关 键 词：针刺 力学信号传导 CSK ATP PCNA 

分 类 号：R245[医药卫生—针灸推拿学]