上海市11种常规粳稻抗稻瘟病基因分子标记检测  

Molecular marker detection of rice blast resistance genes in 11 Shanghai conventional japonica varieties

作　　者：谢水锋 宗士鹏 余飞宇 赵国超[1] 李建粤[1] XIE Shuifeng;ZONG Shipeng;YU Feiyu;ZHAO Guochao;LI Jianyue(College of Life Sciences,Shanghai Normal University,Shanghai 200234,China;Agricultural Technology Center of Guangming Rice(Group)Co.,Ltd.,Shanghai 202171,China)

机构地区：[1]上海师范大学生命科学学院,上海200234 [2]光明米业(集团)有限公司农业技术中心,上海202171

出　　处：《上海师范大学学报（自然科学版）》2022年第1期56-63,共8页Journal of Shanghai Normal University(Natural Sciences)

基　　金：上海市科委现代农业科技创新项目(19391900500)。

摘　　要：利用分子标记检测技术,对9种参加2020年上海市水稻区域试验的品种和2种本课题组新培育的新品系的共10个抗稻瘟病基因位点进行了检测.结果显示,Pi37,Pi41,Pi-d23个基因在11种水稻中出现的频率达100%,Pi2,Pi5,Pi9,Pi36,Pikm和Pib抗性基因在11种水稻中出现的频率分别为18.18%,9.09%,36.36%,90.90%,9.09%和81.81%.此外,11种水稻中都没有检测到Pigm抗性基因.研究结果为聚合更多抗稻瘟病基因,培育广谱抗稻瘟病水稻新品种提供了一定的科学依据.In this study,by using these known molecular markers together with PCR technology,10 rice blast resistance gene loci were amplified in 9 new rice varieties that were involved in 2020 Shanghai Regional Trial and 2 rice lines that were developed in our laboratory.Our results indicated that the occurrence frequency of Pi37,Pi41,and Pi-d2 genes was 100%,while the distribution frequency of Pi2,Pi5,Pi9,Pi36,Pikm,Pib and Pigm genes in 11 tested rice varieties/lines was 18.18%,9.09%,36.36%,90.9%,9.09%,81.81%,and 0,respectively.Collectively,the results of this study provided scientific evidence for pyramiding more blast resistant genes and breeding new rice varieties with broad-spectrum rice blast resistance.

关 键 词：水稻(Oryza sativa) 抗稻瘟病基因 分子标记 

分 类 号：S511[农业科学—作物学]