A Bioorthogonal-Activated Fluorescence Turn-On Probe Based on Nitrone-Modified 1,8-Naphthalimide for Live-Cell Imaging  

作　　者：Yu Teng Hong Yang Xiang Li Yongcheng Wang Dali Yin Yulin Tian 

机构地区：[1]State Key Laboratory of Bioactive Substances and Function of Natural Medicine,Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation,Institute of Materia Medica,Peking Union Medical College and Chinese Academy of Medical Sciences,1st Xian Nong Tan Street,Beijing 100050,China

出　　处：《Chinese Journal of Chemistry》2022年第2期209-214,共6页中国化学（英文版）

基　　金：This work was supported by the Beijing Nova Program(Z201100006820049);the National Natural Science Foundation of China(No.21907109).

摘　　要：A nitrone-modified 1,8-naphthalimide was desig ned as a novel bioorthog on alactivated turn-on probe based on strain-promoted alkyne-nitrone cycloadditio n(SPANC).The bioorthog onal cycloadducts were subseque ntly tran sformed into fluoresce nt rearra nge-ment products by photo-accelerati on,which exhibited sign ificant fluoresce nee enhan ceme nt,large stokes shift,and high fluores-cence qua ntum yield.DFT calculati ons were performed to elucidate the fluoresce nee OFF-ON mecha nism.This fluoroge nic strategy was successfully applied to labeling of proteins and visualizing mitochondria in live cells in real time.

关 键 词：Bioorthogonal reaction FLUORESCENCE NAPHTHALIMIDE Imaging agents CYCLOADDITION 

分 类 号：O657.3[理学—分析化学]