Photoelectrochemical Biosensor for 5-Formylcytosine Based on WS_(2)/Bi/Bi_(2)O_(2)CO_(3) Nanocomposite and Rolling Circle Amplification  

作　　者：Qian Wang Huanshun Yin Yunlei Zhou Lulu Cao Zhengkun Yu Yamin Xu Shiyun Ai 

机构地区：[1]College of Chemistry and Material Science,Food Safety Analysis and Test Engineering Technology Research Center of Shandong Province,Shandong Agricultural University,Taian,Shandong 271018,China

出　　处：《Chinese Journal of Chemistry》2022年第2期247-255,共9页中国化学（英文版）

基　　金：This work was supported by the Outstanding Youth Innova-tion Team of University in Shandong Province(2019KJC019);the Key Research and Development(Public Welfare)Project of Shandong Province(2019GSF107023);the National Natural Science Foundation of China(21775090,41807484,41977345,42077363);the Natural Science Foundation of Shandong Provinee of China(ZR2018MB028).

摘　　要：As a key epigenetic modification,5-formylcytosine(5fC)controls many important cell functions,such as gene expression and cell differentiation.However,5fC exists in mammalian tissues and cells with low content,and its structure is highly similar to 5-methylcytosine(5mC),5-hydroxymethylcytosine(5hmC)and 5-carboxycytosine(5caC), which requires the detection method for detecti ng 5fC with high sensitivity and specificity.In this experiment,WS_(2)/Bi/Bi_(2)O_(2)CO_(3) was used as the photoactive material,5fC antibody was used as the target molecule recog nition reage nt,and rolling amplification(RCA)was employed as the signal amplification strategy to con struct a no vel photoelectrochemical biose nsor for detecti ng 5-formylcytosi ne deox ynu cleotide(SfdCTP).

关 键 词：Semicon ductors PHOTOELECTROCHEMISTRY BIOSENSOR Epigenetic modification TOXICOLOGY 

分 类 号：TB383[一般工业技术—材料科学与工程] O646[理学—物理化学]