Synergistic combination of colistin with imipenem, amikacineor ciprofloxacin against Acinetobacter baumannii andPseudomonas aeruginosa carbapenem-resistant isolated inAnnaba hospital Algeria  

作　　者：SAIDA MELIANI SOUMAYA TOUMI HEYTHEM DJAHOUDI KHALED DEGHDEGH KAMEL AMOURA ABDELGHANI DJAHOUDI 

机构地区：[1]Department of Biochemistry,Faculty of Sciences,Laboratory of Microbiology,Badji Mokhtar University,Annaba,Algeria [2]Department of Biochemistry,Eco-biology Laboratory for Marine and Coastal Areas,Faculty of Sciences,BP 12 El-Hadjar,BadjiMokhtar University,Annaba,Algeria [3]Faculty of Medicine,Pulmonology Service Badji Mokhtar University,Annaba,Algeria [4]Faculty of Medicine,CHU Annaba,Badji Mokhtar University,Annaba,Algeria [5]Department of Pharmacy,Faculty of Medicine,Laboratory of Microbiology,Badji Mokhtar University,Annaba,BP 205,Algeria

出　　处：《BIOCELL》2020年第2期175-182,共8页生物细胞（英文）

摘　　要：Objective:The aim of this study is to detect in vitro the synergetic activity of colistin in combination with imipenem,amikacin or ciprofloxacin,at sub-inhibitory concentrations,against carbapenems-resistant(CR)Acinetobacter baumannii and Pseudomonas aeruginosa strains isolated from various wards in Annaba teaching hospital in eastern Algeria.Materials and Methods:The minimal inhibitory concentrations(MIC)were determined by broth macrodilution(BMD).Carbapenemase encoding genes were screened using polymerase chain reaction(PCR).The activity of colistin in combination with second antibiotic was evaluated by the Checkerboard Technique.Results:39 CR P.aeruginosa and 21 CR A.baumanni strains where collected.The MIC values ranging from(0.25 to 4μg/ml)to colistin,≥16μg/ml for imipenem,≥4μg/ml to amikacin and≥8μg/ml ciprofloxacin.The PCR reveals the presence of the genes blaOXA23(n=12),blaOXA24(n=6),blaNDM1(n=3)in A.baumannii and blaVIM2(n=12)in P.aeruginosa.The combination of colistin with imipenem showed synergistic effect on 57.14%and 46.15%of A.baumannii and P.aeruginosa isolates,respectively.For colistin and amikacin,the synergistic effect is detected in 28.6%of A.baumannii and 30.8%of P.aeruginosa.While colistin and ciprofloxacin showed synergy on 14.29%and 15.38%of A.baumannii and P.aeruginosa isolates,respectively.Conclusion:CR A.baumannii and P.aeruginosa remain the most prevalent infection agents in patients from high-risk wards at Annaba Hospital.Colistin associated with imipenem or with amikacin at sub-inhibitory concentrations gives very encouraging results allowing better management of infections caused by this type of bacteria.

关 键 词：CARBAPENEMASES COLISTIN COMBINATION GNB-NF Sub-inhibitory concentration 

分 类 号：R37[医药卫生—病原生物学]