实现微米级灭菌范围控制的微细等离子体射流装置  

Micro plasma jet devices for micron sterilization range control

作　　者：张雨晗 朱鸿成 杜晓霞 肖文香[1] 李华[1] ZHANG Yuhan;ZHU Hongcheng;DU Xiaoxia;XIAO Wenxiang;LI Hua(School of Life and Environmental Sciences,Guilin University of Electronic Technology,Guilin 541004,China)

机构地区：[1]桂林电子科技大学生命与环境科学学院,广西桂林541004

出　　处：《光学精密工程》2022年第3期296-309,共14页Optics and Precision Engineering

基　　金：国家自然科学基金资助项目(No.61864001,No.62163009);广西自然科学基金重点项目(No.2021GXNSFDA196005);广西自动检测技术与仪器重点实验室主任基金资助项目(No.YQ21111);广西研究生教育创新计划项目(No.YCSW2021181)。

摘　　要：为了实现微细等离子体的精准灭菌,设计了新型微细等离子体射流装置,并对该装置产生的含氧活性粒子(Reactive Oxygen Species,ROS)和含氮活性粒子(Reactive Nitrogen Species,RNS)分布范围及其灭菌范围进行研究。淀粉碘化钾混合溶液里的碘离子可以被微细等离子体射流产生的ROS,RNS氧化成碘单质,根据淀粉遇碘变蓝的显色原理,使用含有淀粉碘化钾混合溶液的琼脂培养基表征该装置射流中ROS,RNS的分布范围。将菌液涂布在琼脂培养基上,使用微细等离子体射流装置在相同的条件下处理不同的时间,于生化培养箱中37℃培养以进行灭菌范围的表征。最后在Ts2FL尼康倒置荧光显微镜下进行观察。实验结果表明,在交流电压幅值为5 kV,中心频率为10 kHz,作用时间为10,20和30 s时,灭菌范围分别控制在30,65和75μm的直径内。等离子体射流与处理物体表面不直接接触和等离子体与物体表面直接接触两种作用方式相比,前者的灭菌范围更小,更容易实现精准控制。该装置将目前毫米量级的灭菌范围提高到了微米量级,对等离子体医学研究具有重要意义。In order to achieve precise sterilization using micro plasma,a novel micro plasma jet device was designed,and the distribution range of the reactive oxygen species(ROS)and reactive nitrogen species(RNS)generated by the device and the sterilization range of the device were investigated.First,iodine ions(I-)in a potassium starch iodide mixture were oxidized to iodine monomers(I2)by the ROS and RNS generated by microplasma jets.The range of distribution of the ROS and RNS in the jet of the device was characterized using an agar medium containing the starch-potassium iodide mixture,based on the color development principle of starch,which turns blue when exposed to iodine.Next,a bacterial solution was coated on an agar medium and incubated overnight at 37°C in a biochemical incubator using a micro plasma jet device for different times under the same conditions for characterization of the sterilization range.Finally,the sterilization ranges were observed under a Ts2 FL Nikon inverted fluorescence microscope.The experimental results indicate that the sterilization range can be controlled within diameters of 30,65,and75μm at an AC voltage amplitude of 5 kV,a center frequency of 10 kHz,and an action time of 10,20,and 30 s,respectively.A plasma jet does not directly contact the surface of the object,whereas plasma directly contacts the surface of the object;the former has a smaller sterilization range,and it is easier to achieve accurate control.This device improves the current millimeter-scale sterilization range accuracy to the micron scale,which is of great significance for plasma medical research.

关 键 词：微细等离子体 灭菌 淀粉碘化钾混合溶液 微米级 

分 类 号：TM213[一般工业技术—材料科学与工程]