非对称截面螺旋流道中微粒的惯性聚焦效应  

Inertial focusing effect of particles in spiral microchannel with asymmetric cross-section

作　　者：韩帅 张鑫杰 顾乔 刘尧 王昕怡 HAN Shuai;ZHANG Xinjie;GU Qiao;LIU Yao;WANG Xinyi(College of Mechanical and Electrical Engineering,Hohai University,Changzhou 213022,China;Gynecology and Obstetrics Department,The Third Affiliated Hospital of Soochow University,Changzhou 213000,China)

机构地区：[1]河海大学机电工程学院,江苏常州213022 [2]苏州大学附属第三医院妇产科,江苏常州213000 [3]河海大学机电工程学院智能制造研究所,江苏常州213022

出　　处：《光学精密工程》2022年第3期310-319,共10页Optics and Precision Engineering

基　　金：国家自然科学基金资助项目(No.51905150);江苏省自然科学基金资助项目(BK20190167);中央高校基本科研业务费资助项目(No.B200202230);江苏省博士后科研资助计划项目(No.2019K033)。

摘　　要：为实现生物微粒/细胞的精确操控,提出了一种非对称截面螺旋流道结构的惯性微流控芯片。基于仿真和实验的方法,对不同尺寸微粒在微流道中的惯性聚焦行为进行了研究。设计了一种"L"形截面的螺旋流道,采用仿真软件COMSOL研究微流道中的二次流场及微粒的运动轨迹。使用UV激光切割与等离子清洗键合的工艺制作芯片样件,采用高速摄像机和荧光显微镜分别拍摄6,10和15μm粒子在微流道中不同流量时的运动轨迹。最后,对粒子运动图片进行堆叠分析,研究微粒的惯性聚焦迁移机理。结果表明:"L"形截面中产生了两对强度不同的非对称二次流场,使得10μm和15μm粒子在微流道外圈实现了强聚焦,而6μm粒子实现了粗聚焦。该研究表明利用非对称二次流可以调节微粒的聚焦位置,为微粒和细胞的精准操控提供新的思路。An inertial microfluidic chip with a spiral microchannel of asymmetric cross-section was proposed to achieve a precise control of biological microparticles/cells.The inertial focusing behavior of particles of different sizes in the microchannel was studied through simulation and experiment.A spiral channel with L shaped cross-section was designed,and the secondary flow field distribution and particle trajectory in the channel were analyzed using COMSOL simulation software.The prototype chip was fabricated by UV laser cutting and plasma cleaning bonding.The trajectories of particles of sizes 6,10,and 15 μm at different flow rates in the channel were captured by a high speed camera and fluorescent microscope.Finally,the images of particle trajectories were stacked and analyzed and the inertial focusing and migration mechanism of the particles were investigated.The results show that two asymmetric secondary flow vortexes of different strengths are produced in the L shaped cross-section.Furthermore,the particles of 10 and 15 μm sizes focus tightly in the outer ring of the microchannel,whereas those of 6 μm size focus in it roughly.The particle focusing position can be adjusted using asymmetric secondary flow,thus providing new insights into precise particle and cell manipulation.

关 键 词：微流控 非对称二次流 惯性聚焦 微粒操控 激光加工 

分 类 号：O352[理学—流体力学]