广东紫珠中的萜类化合物及其抗炎活性  

Terpenoids from Callicarpa kwangtungensis and their anti-inflammatory activity

作　　者：黄逸敏 袁欢[1] 陈萍 王涛[1] 彭光天[1] 吴爱芝[1] HUANG Yi-min;YUAN Huan;CHEN Ping;WANG Tao;PENG Guang-tian;WU Ai-zhi(School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006,China)

机构地区：[1]广州中医药大学中药学院,广州510006

出　　处：《天然产物研究与开发》2022年第1期50-56,共7页Natural Product Research and Development

基　　金：国家自然科学基金(82073986)。

摘　　要：对岭南药材广东紫珠(Callicarpa kwangtungensis)地上部分进行化学成分研究,得到11个萜类化合物,分别鉴定为sambucunlin A(1)、2α-羟基羽扇豆醇(2)、swinhoeic acid(3)、3β-羟基-乌苏烷-11-烯-13β,28-内酯(4)、蔷薇酸(5)、2α,3β,6β,18β,23-pentahydroxy-olean-12-en-28-oic acid(6)、rel-5-(3 S,8 S-dihydroxy-1 R,5 S-dimethyl-7-oxa-6-oxobicyclo-oct-8-yl)-3-methyl-2 Z,4 E-pentadienoic acid(7)、salvionoside B(8)、齐墩果酸(9)、白桦脂酸(10)和α-香树脂醇(11)。其中,化合物1~4和6~8为首次从该属植物中分离得到。在化学成分分离基础上,进一步选择脂多糖(LPS)诱导的RAW 264.7小鼠巨噬细胞炎症模型进行萜类化合物抗炎活性测试。结果表明:化合物3和9具有显著的抗炎活性,对比结构发现,三萜类化合物(3~6、9和11)抗炎活性优于倍半萜类化合物(7和8)。研究结果广东紫珠萜类成分在抗炎方面的临床应用提供了实验依据。Eleven terpenoids were isolated and purified from the ethanol extract of Callicarpa kwangtungensis of Lingnan Herbs.Based on spectral analysis and the comparition of literatures,these compounds were identified as sambucunlin A(1),2α-hydroxy-lupeol(2),swinhoeic acid(3),3β-hydroxy-urs-11-en-13β,28-olide(4),euscaphic acid(5),2α,3β,6β,18β,23-pentahydroxy-olean-12-en-28-oic acid(6),rel-5-(3 S,8 S-dihydroxy-1 R,5 S-dimethyl-7-oxa-6-oxobicyclo-oct-8-yl)-3-methyl-2 Z,4 E-pentadienoic acid(7)and salvionoside B(8),oleanolic acid(9),betulinic acid(10),α-amyrin(11),respectively.Compounds 1-4,6-8 was firstly isolated from the genus of Callicarpa.Further the lipopolysaccharide(LPS)-induced RAW 264.7 mouse macrophages was explored as inflammatory model to measure the anti-inflammatory activity of the terpenoids.The experimental results revealed that compounds 3 and 9 had obvious anti-inflammatory activity.It was found that the anti-inflammatory activity of triterpenoids(3-6,9 and 11)was better than that of sesquiterpenoids(7 and 8).This study provided the experimental basis for the clinical application of C.kwangtungensis in anti-inflammatory fields.

关 键 词：马鞭草科 广东紫珠 萜类 抗炎 

分 类 号：R284[医药卫生—中药学]