羊角拗茎的化学成分及其抗炎活性研究  

Study on chemical constituents and their anti-inflammatory activity from stems of Strophanthus divaricatus

作　　者：于淼 冉红玲 梅文莉[1,2] 黄圣卓 魏艳梅[1] 蔡彩虹 戴好富 王昊[1,2] YU Miao;RAN Hong-ling;MEI Wen-li;HUANG Sheng-zhuo;WEI Yan-mei;CAI Cai-hong;DAI Hao-fu;WANG Hao(Key Laboratory of Natural Products Research and Development from Li Folk Medicine of Hainan Province,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences;Key Laboratory of Conservation and Utilization of Tropical Agro-bioresources of Hainan Province,Hainan Academy of Tropical Agricultural Resources,Haikou 571101,China)

机构地区：[1]中国热带农业科学院热带生物技术研究所海南省黎药资源天然产物研究与利用重点实验室 [2]海南热带农业资源研究院海南省热带农业生物资源保护与利用重点实验室,海口571101

出　　处：《天然产物研究与开发》2022年第1期57-62,共6页Natural Product Research and Development

基　　金：2020年海南省自然科学基金青年基金(320QN334);中国热带农业科学院院级创新团队基本科研业务费(1630052017005)。

摘　　要：探讨羊角拗茎的化学成分及其抗炎活性,采用硅胶、Sephadex LH-20等色谱分离技术分离纯化羊角拗茎乙醇提取物的乙酸乙酯萃取物中的化合物,通过质谱和核磁共振等波谱技术对其结构进行鉴定,确定出了13个化合物,包括常春藤皂苷元(1)、(-)-loliolide(2)、(3 S,5 R,6 S,7 E)-3,5,6-trihydroxy-7-megastigmen-9-one(3)、(3 R,6 R,7 E)-3-hydroxy-4,7-megastigmadien-9-one(4)、松脂素(5)、表松脂素(6)、4,4′-dihydroxy-3,3′-dimethoxybenzophenone(7)、6-羟基柚皮素(8)、东莨菪素(9)、6-羟基-7,8-二甲氧基香豆素(10)、吲唑(11)、香草酸(12)、对羟基苯甲酸(13)。其中化合物1~4和6~13为首次从羊角拗中分离得到。采用LPS诱导小鼠单核巨噬细胞RAW 264.7体外细胞炎症模型测试结果表明,化合物5能显著抑制NO的产生,IC_(50)值为18.09±1.09μM。To study the chemical constituents and their anti-inflammatory activity from the stems of Strophanthus divaricatus,thirteen compounds were isolated from its ethyl acetate fraction of ethanol extract by using chromatographic techniques including silica gel,Sephadex LH-20 etc.Their structures were elucidated on the basis of spectroscopic data analysis as hederagenin(1),(-)-loliolide(2),(3 S,5 R,6 S,7 E)-3,5,6-trihydroxy-7-megastigmen-9-one(3),(3 R,6 R,7 E)-3-hydroxy-4,7-megastigmadien-9-one(4),(+)-pinoresinol(5),epipinoresinol(6),4,4′-dihydroxy-3,3′-dimethoxybenzophenone(7),6-hydroxynaringenin(8),scosolrtin(9),6,7-dimethylesculetin(10),indazole(11),vanillic acid(12),and p-hydroxybenzoic acid(13).Compounds 1~4 and 6~13 were identified from this plant for the first time.Compound 5 showed a significant inhibition on the lipopolysaccharide(LPS)-induced nitric oxide(NO)production in RAW 264.7 cells with IC_(50) value of 18.09±1.09μM.

关 键 词：羊角拗 化学成分 抗炎活性 

分 类 号：R284.2[医药卫生—中药学]