基于正交实验法优化从栀子黄色素中制备藏红花酸的工艺  

Optimization of preparation process of crocetin from gardenia yellow pigment by orthogonal experiment

作　　者：阳志强 乌兰[1] 李茂星 李晓琳 韩天翔 王维刚 王芃 YANG Zhi-qiang;WU Lan;LI Mao-xing;LI Xiao-lin;HAN Tian-xiang;WANG Wei-gang;WANG Peng(Institute of Chemical Technology,Northwest Minzu University,Lanzhou 730030,China;Department of Clinical Pharmacy,the 940th Hospital of Joint Logistic Support Force of PLA;Gansu Plateau Pharmaceutical Technology Center,Lanzhou 730050,China;School of Pharmacy,Gansu University of Chinese Medicine,Lanzhou 730000,China)

机构地区：[1]西北民族大学化工学院,兰州730030 [2]联勤保障部队第九四〇医院临床药学科 [3]甘肃省高原药学行业技术中心,兰州730050 [4]甘肃中医药大学药学院,兰州730000

出　　处：《天然产物研究与开发》2022年第1期76-82,共7页Natural Product Research and Development

基　　金：甘肃省重点人才项目(2021-0623-RCC-0038);中央高校基本科研业务费专项资金(31920200002);后勤科研项目(GLB21J036);拔尖培育项目(2021yxky001)。

摘　　要：基于正交实验法,优化从栀子黄色素中提取制备藏红花酸的碱水解工艺,以期可以简单高效地获得高纯度藏红花酸。建立高效液相色谱法(HPLC)测定藏红花酸含量,以藏红花酸的含量和得率为考察指标,采用正交实验法考察碱水解工艺中的料液比、NaOH浓度、水解温度和水解时间对产品中藏红花酸含量和得率的影响。确定栀子黄色素碱水解的最佳条件:料液比1∶6 g/mL、NaOH浓度3 mol/L、水解温度55℃、水解时间60 min。在该条件下制备获得的藏红花酸得率可达15.33%±1.25%;含量可达到97.24%±0.78%。优化后方法步骤简单易行,绿色无污染,一步制得高纯度藏红花酸,适用于工业化生产。To simply and efficiently prepare high-purity crocetin from gardenia yellow pigment,the alkaline hydrolysis process was optimized using an orthogonal experimental design.High-performance liquid chromatography(HPLC)was established for the determination of crocetin in the samples.The evaluation index was based on the purity and yield of crocetin.The solid-liquid ratio,NaOH concentration,hydrolysis temperature,and hydrolysis time were investigated.The optimal conditions for alkaline hydrolysis of gardenia yellow pigment were obtained as follows:solid-liquid ratio 1∶6 g/mL,NaOH concentration 3 mol/L,hydrolysis temperature 55°C,and hydrolysis time 60 min.Under this condition,the crocetin yield was 15.33%±1.25%and a content of 97.24%±0.78%.Finally,high-purity crocetin was prepared from the gardenia yellow pigment in a single step.This method is simple,environmentally friendly,and suitable for industrial application.

关 键 词：藏红花酸 栀子黄色素 碱水解 高效液相色谱法 正交实验 

分 类 号：R284.2[医药卫生—中药学]