基于多元统计分析的酒制前后女贞子成分差异分析  

Analysis of components of raw and wine-processed Ligustri Lucidi Fructus based on multivariate statistical analysis

作　　者：李焕茹 冯志毅[1,2,3] 赵迪 胡雁萍[1,2,3] 李柯 冯素香 LI Huan-ru;FENG Zhi-yi;ZHAO Di;HU Yan-ping;LI Ke;FENG Su-xiang(Henan University of Chinese Medicine;Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-constructed by Henan province&Education Ministry of P.R.China;Zhengzhou Key Laboratory of Chinese Medicine for Quality Control and Evaluation,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学 [2]呼吸疾病中医药防治省部共建协同创新中心 [3]郑州市中药质量控制与评价重点实验室,郑州450046

出　　处：《天然产物研究与开发》2022年第1期102-113,共12页Natural Product Research and Development

基　　金：河南省科技攻关项目(142102310280)。

摘　　要：为探究酒制对女贞子化学成分的影响,采用UPLC-Orbitrap-MS技术结合多元统计分析对生女贞子、酒女贞子差异性化学成分进行研究。基于分子网络技术、主成分分析(PCA)模型和正交偏最小二乘法-判别分析(OPLS-DA)模型筛选女贞子酒制前后出现差异的主要化学成分。共鉴定出42种化学成分,结果显示PCA,OPLS-DA可将女贞子与酒女贞子完全区分开来,OPLS-DA筛选出VIP>1的22个酒制前后出现差异的主要化学成分。本研究建立了一种新的数据处理策略,以系统识别生女贞子和酒女贞子的化学成分,筛选女贞子酒制前后的差异性成分,为进一步研究女贞子酒制增效物质基础及原理提供坚实的基础。In order to explore the effect of wine processing on the chemical components of Ligustri Lucidi Fructus,UPLC-Orbitrap-MS technology combined with multivariate statistical analysis was used to study the differential chemical components of raw Ligustri Lucidi Fructus(RLLF)and wine-processed Ligustri Lucidi Fructus(WLLF).The molecular network technology,principal component analysis(PCA)model and orthogonal partial least squares-discriminant analysis(OPLS-DA)model were used to select the main chemical components that showed differences before and after the wine processing of Ligustri Lucidi Fructus.A total of 42 compounds were identified,and the results showed that there were obvious distinction between RLLF and WLLF.Twenty-two chemical components showing differences before and after wine processing were selected out by OPLS-DA with VIP>1 before and after wine processing.In this study,a new data processing strategy was established to systematically identify the chemical components of RLLF and WLLF,and to select the different components before and after wine processing of RLLF,which provided a solid foundation for further study on the material basis and principle of enhancing the efficiency of wine processing of RLLF.

关 键 词：女贞子 UPLC-Orbitrap-MS 化学成分 酒制 分子网络 

分 类 号：R284.1[医药卫生—中药学]