抗菌肽对育成期母貂生长性能、养分表观消化率及肠道菌群的影响  

Effects of Antimicrobial Peptide on Growth Performance,Nutrient Apparent Digestibilities and Intestinal Flora of Growing Female Minks

作　　者：宇晓军 李丹丹 王路义 郭贽豪 王光[1] 王利华[1] YU Xiaojun;LI Dandan;WANG Luyi;GUO Zhihao;WANG Guang;WANG Lihua(College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China)

机构地区：[1]青岛农业大学动物科技学院,青岛266109

出　　处：《动物营养学报》2022年第2期1194-1204,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：山东省现代农业产业技术体系特种经济动物创新团队(SDAIT-21-8)。

摘　　要：本试验旨在探究抗菌肽对育成期母貂生长性能、养分表观消化率及肠道菌群的影响。选取65日龄体重相近的短毛黑母貂60只,随机分为6组,每组10个重复,每个重复1只。各组饲粮中分别添加0(对照组)、100、200、300、400、500 mg/kg的抗菌肽(天蚕素活性多肽)。预试期1周,正试期8周。结果表明:1)与对照组相比,200 mg/kg抗菌肽添加组末重、平均日增重显著升高(P<0.05),100、200、400、500 mg/kg抗菌肽添加组体长显著升高(P<0.05)。2)与对照组相比,200、300、400 mg/kg抗菌肽添加组1~5周粗蛋白质表观消化率显著升高(P<0.05),100、200、300、400 mg/kg抗菌肽添加组6~8周粗脂肪表观消化率显著升高(P<0.05)。3)与对照组相比,200、300 mg/kg抗菌肽添加组Ace指数显著升高(P<0.05),300 mg/kg抗菌肽添加组Chao1指数显著升高(P<0.05)。在门水平上,与对照组相比,100、200 mg/kg抗菌肽添加组厚壁菌门相对丰度显著升高(P<0.05),100、200、300 mg/kg抗菌肽添加组变形菌门相对丰度显著降低(P<0.05)。在属水平上,与对照组相比,200、300 mg/kg抗菌肽添加组乳酸菌属相对丰度显著升高(P<0.05),400 mg/kg抗菌肽添加组伯克霍尔德菌属相对丰度显著升高(P<0.05),100、200、300、400和500 mg/kg抗菌肽添加组梭菌属相对丰度显著降低(P<0.05)。综合以上指标,在本试验条件下,饲粮中添加100、200 mg/kg抗菌肽可提高育成期母貂末重、平均日增重、体长以及粗蛋白质和粗脂肪表观消化率,调节菌群多样性,提高厚壁菌门、乳酸菌属相对丰度,降低变形菌门、梭菌属相对丰度。The present experiment was conducted to explore the effects of antimicrobial peptide on growth performance,nutrient apparent digestibilities and intestinal flora of growing female minks. Sixty short-haired black female minks with similar body weight at 65 days of age were selected and divided into 6 groups with 10 replicates per group and 1 mink per replicate. Minks in six groups were fed basal diets supplemented with 0(control group),100,200,300,400 and 500 mg/kg antimicrobial peptide,respectively. The pre-test lasted for 1 week,and the experiment lasted for 8 weeks. The results showed as follows:1)compared with the control group,the final weight and average daily gain of 200 mg/kg antimicrobial peptide addition group were significantly increased(P<0.05),and the body length of 100,200,400 and 500 mg/kg antimicrobial peptide addition groups was significantly increased(P<0.05). 2)Compared with the control group,the crude protein apparent digestibility during 1 to 5 weeks of 200,300 and 400 mg/kg antimicrobial peptide addition groups was significantly increased(P<0.05),and the ether extract apparent digestibility during 6 to 8 weeks of 100,200,300 and 400 mg/kg antimicrobial peptide addition groups was significantly increased(P<0.05). 3)Compared with the control group,the Ace index of 200 and 300 mg/kg antimicrobial peptide addition groups was significantly increased(P<0.05),and the Chao1 index of 300 mg/kg antimicrobial peptide addition group was significantly increased(P<0.05). At the phylum level,compared with the control group,the Firmicutes relative abundance of 100 and 200 mg/kg antimicrobial peptide addition groups was significantly increased(P<0.05),and the Proteobacteria relative abundance of 100,200 and 300 mg/kg antimicrobial peptide addition groups was significantly decreased(P<0.05). At the genus level,compared with the control group,the Lactobacillus relative abundance of 200 and 300 mg/kg antimicrobial peptide addition groups was significantly increased(P<0.05),the Burkholderia relative abundance of

关 键 词：抗菌肽 水貂 生长性能 养分表观消化率 肠道菌群 

分 类 号：S865.22[农业科学—野生动物驯养]