稀释法对抗核抗体中复合核型结果判读的影响  

Effect of dilution method on the interpretation of multi-karyotypes in anti-nuclear antibody

作　　者：钱红 朱文波[2] 陶月[2] 宁明哲[2] 戴蕾[2] QIAN Hong;ZHU Wenbo;TAO Yue;NING Mingzhe;DAI Lei(Department of Clinical Laboratory,Suqian Hospital of Nanjing Drum Tower Hospital Group,Jiangsu Province,Suqian 223800,China;Department of Clinical Laboratory,Drum Tower Hospital Affiliated to Nanjing University Medical College,Jiangsu Province,Nanjing 210008,China)

机构地区：[1]南京鼓楼医院集团宿迁医院检验科,江苏宿迁223800 [2]南京大学医学院附属鼓楼医院检验科,江苏南京210008

出　　处：《中国当代医药》2022年第5期136-138,147,F0003,共5页China Modern Medicine

摘　　要：目的探讨稀释法对抗核抗体(ANA)中复合核型结果判读的影响。方法收集2019年6月至8月在南京鼓楼医院就诊患者的56例细胞核复合核型标本,10例细胞核单核型标本,采用免疫印迹法检测抗核抗体谱(IgG),间接免疫荧光法按照1∶100、1∶1000、1∶10000稀释检测抗核抗体免疫球蛋白G(IgG),判断荧光可见的最高稀释滴度,结合ANA谱的结果比较稀释前后核型及滴度判读结果的差异。结果稀释前后,10例细胞核单核型标本的核型及滴度均无变化。在56例细胞核复合核型标本中,分析核型,增多的有8例(14.2%),减少的有3例(5.4%),不变的有45例(80.4%);分析滴度,升高的有23例(41.1%),降低的有8例(14.2%),部分升高部分降低的有2例(3.6%),不变的有23例(41.1%),该结果与ANA谱的结果相关性更强。结论当出现细胞核复合核型时,可以通过稀释的方法来协助判断核型及滴度,从而提高结果的准确性。Objective To explore the effect of dilution method on the interpretation of multi-karyotypes in anti-nuclear antibody(ANA).Methods From June to August 2019,56 nuclear composite karyotypes and 10 nuclear monokaryotypes from patients admitted to Nanjing Drum Tower Hospital were collected.Western blotting was used to detect anti-nuclear antibody(IgG)spectra,and indirect immunofluorescence method was used to detect anti-nuclear antibody immunoglobulin G(IgG)diluted at 1∶100,1∶1000,and 1∶10000.The maximum dilution titer of fluorescence was determined,and the differences of karyotype and titer interpretation results before and after dilution were compared combined with ANA spectrum results.Results There was no change in karyotype and titer of 10 nuclear mono-karyotype samples before and after dilution.Among 56 nuclear compound karyotypes,8 cases(14.2%)were increased,3 cases(5.4%)were decreased,and 45 cases(80.4%)remained unchanged.The titer analysis showed that 23 cases(41.1%)were increased,8 cases(14.2%)were decreased,2 cases(3.6%)were partially increased and partially decreased,and 23 cases(41.1%)had no change.This result was more correlated with ANA spectrum results.Conclusion The method of dilution can help to determine the karyotypes and titers of nuclear multi-karyotypes,so as to improve the accuracy of the results.

关 键 词：抗核抗体 复合核型 单核型 稀释 

分 类 号：R446.6[医药卫生—诊断学]