超声辅助提取柚皮白瓤黄酮工艺的响应曲面优化  

Optimization of Response Surface for Ultrasonic-assisted Extraction of Flavonoid from Pomelo Peel White Flesh

作　　者：张倩倩 李伟 田成 陈业 莫开菊 ZHANG Qianqian;LI Wei;TIAN Cheng;CHEN Ye;MO Kaijü(College of Biological Science and Technology,Hubei Minzu University,Enshi 445000,China;Hubei Key Laboratory of Biological Resources Protection and Utilization,Enshi 445000,China)

机构地区：[1]湖北民族大学生物科学与技术学院,湖北恩施445000 [2]生物资源保护与利用湖北省重点实验室,湖北恩施445000

出　　处：《湖北民族大学学报（自然科学版）》2022年第1期26-32,共7页Journal of Hubei Minzu University:Natural Science Edition

基　　金：湖北省科技攻关计划项目(2010BBB016)。

摘　　要：深入开发利用湖北恩施白柚资源,探究超声波对白柚柚皮白瓤中黄酮提取率的影响,并对提取工艺参数进行优化,为恩施白柚柚皮成分的有效提取和利用提供参考.通过单因素实验探讨超声功率、超声提取时间、超声频率对柚皮黄酮提取率的影响,采用响应面中Box-Behnken法对黄酮提取工艺进行优化,响应面模型P=0.004 5<0.01,失拟项P=0.265 7>0.05,数据与模型拟合较好.结果得出:最优提取参数条件组合为超声时间1.518 h、超声功率为646.075 W、超声频率为79.819 kHz,在该条件下黄酮提取率可达到1.117%;结合实际情况经试验验证,采用超声时间1.5 h,超声功率640 W、超声频率80 kHz时黄酮提取率可达1.14%,模型与实际结果仅相差0.023%,优化效果良好.In order to develop and utilize the resources of flavonoids in Enshi white pomelo, we explore the effect of ultrasound on the extraction rate of flavonoids from white flesh of pummelo peel.The extraction parameters were optimized to provide reference for effective extraction and utilization of pummelo peel resources of Enshi white pomelo.The effects of ultrasonic power(W),ultrasonic extraction time(h) and ultrasonic frequency(kHz) on the extraction yield of flavonoids from pomelo peel were investigated by single factor experiment.The Box-Behnken method in the response surface was used to optimize the extraction process of flavonoids.The response surface model P=0.004 5<0.01 and the lost fit term P=0.265 7>0.05 were well fitted with the model.The results showed that the optimal extraction parameters were as follows: ultrasonic time of 1.518 h, ultrasonic power of 646.075 W and ultrasonic frequency of 79.819 kHz.Under these conditions, the extraction yield of flavonoids could reach 1.117%.Combined with the actual situation, the experiment verified that the extraction rate of flavonoids could reach 1.14% when the ultrasonic time was 1.5 h, the ultrasonic power was 640 W and the ultrasonic frequency was 80 kHz.The difference between the model and the actual result was only 0.023%,and the optimization effect was good.

关 键 词：柚皮白瓤 超声波 黄酮 响应面优化 提取工艺 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]