山药块茎发育阶段基因共表达网络构建及阶段特异性分析  

Stage-specific and weighted gene co-expression network analyses of yam(Dioscorea alata L.)tuber growth

作　　者：殷剑美[1] 张铅 蒋璐[1] 张培通[1] YIN Jian-mei;ZHANG Qian;JIANG Lu;ZHANG Pei-tong(Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)

机构地区：[1]江苏省农业科学院经济作物研究所,江苏南京210014

出　　处：《江苏农业学报》2022年第1期30-38,共9页Jiangsu Journal of Agricultural Sciences

基　　金：国家现代农业产业技术体系资助项目(CARS-21)。

摘　　要：山药是一种药食同源作物,块茎是其主要产品器官,其发育机制对于山药产量和品质提升相关育种有着重要的指导作用。以不同生长阶段(块茎膨大第1 d、第11 d、第21 d、第31 d、第41 d、第51 d,T_(1)~T_(6))的山药块茎为材料进行转录组测序,经从头(de novo)组装后获得42042个unigenes,利用七大数据库进行注释,鉴定后发现山药块茎形成过程中涉及大量基因,包括激素合成、信号转导等相关基因。阶段特异性分析结果表明,块茎形成初始阶段为块茎发育的关键时期;加权基因共表达网络分析结果表明,在山药块茎形成初期及后期,激素相关基因的表达特别活跃,脱落酸(ABA)、茉莉酸(JA)是山药块茎生长发育的重要激素。研究结果为进一步研究山药块茎膨大分子机制奠定了基础。Chinese yam is a crop with hamology of medicine and food,and tuber is its main product organ.The development mechanism of tubers plays an important guiding role in the breeding related to the improvement of yield and quality of yam.Based on transcriptome sequencing of tubers at different growth stages(T_(1)-T_(6)),after de novo assembly,42042 unigenes were obtained and annotated using seven databases.After identification,it was found that a large number of genes were involved in the process of tuber formation,including hormone synthesis-related genes,signal transduction-related genes,and so on.The results of stage specificity analysis showed that the initial stage of tuber formation was a critical period.The results based on the weighted gene co-expression network analysis(WGCNA)showed that the expression of hormone-related genes was very active in the early and late stages of tuber growth.Abscisic acid(ABA)and jasmonic acid(JA)were important hormones for the growth and development of yam tubers.The results lay a foundation for further study on the molecular mechanism of tuber enlargement of yam.

关 键 词：山药 转录组测序 阶段特异性基因 基因共表达网络 

分 类 号：S632.101[农业科学—蔬菜学]