利用分子标记辅助选择技术改良三系不育系荃9311A稻瘟病抗性的研究  

Studies on Improving Rice Blast Resistance of CMS Line Quan 9311A by Molecular Marker-assisted Technology

作　　者：张云虎[1] 严志[1] 杨力 李方宝[1] 庞战士[1] 邓一文 王慧[1] 冯旗 张从合 陈琳[1] 王合勤[1] 黄艳玲[1] 刘兴江[1] 申广勒[1] ZHANG Yun-hu;YAN Zhi;YANG Li;LI Fang-bao;PANG Zhan-shi;DENG Yi-wen;WANG Hui;FENG Qi;ZHANG Cong-he;CHEN Lin;WANG He-qin;HUANG Yan-ling;LIU Xing-jiang;SHEN Guang-le(Anhui Win-all Hi-tech Seed Co.,Ltd./National Key Laboratory for New Variety Development of Hybrid Rice,Ministry of Agriculture and Rural Affairs,Hefei,Anfei 230088,China;CAS Center for Excellence in Molecular Plant Sciences/Institute of Plant Physiology and Ecology,Shanghai 200032,China)

机构地区：[1]安徽荃银高科种业股份有限公司/农业部杂交稻新品种创制重点实验室,安徽合肥230088 [2]中国科学院分子植物科学卓越创新中心/植物生理生态研究所,上海200032

出　　处：《杂交水稻》2022年第1期19-24,共6页Hybrid Rice

基　　金：安徽省科技重大专项(201903a06020011)。

摘　　要：为了提高三系不育系荃9311A的稻瘟病抗性,以含广谱稻瘟病抗性基因Pigm的水稻品种9311为供体,保持系荃9311B为受体和轮回亲本,通过分子标记辅助选择技术筛选抗性基因、剔除恢复基因和遗传背景筛选,在稻瘟病病区进行抗性鉴定,人工接种鉴定以及田间农艺性状考查,于BC2F2代获得有Pigm基因并剔除了恢复基因的改良株系,其后再与荃9311A杂交和连续回交,育成了农艺性状与荃9311A无显著差异而稻瘟病抗性明显增强的三系不育系K9311A及其保持系K9311B。To improve rice blast resistance of the CMS line Quan 9311 A,the rice variety 9311 containing the broadspectrum rice blast resistance gene Pigm was used as the donor,and the maintainer line Quan 9311 B was used as the recipient and recurrent parent.By using the molecular marker-assisted selection technology to select the resistance gene,eliminate the restoring genes and conduct genetic background screening,identifying the resistance in the fields of rice blast disease areas,identifying the blast resistance by artificial inoculation,and investigating the agronomic traits in the fields,the improved line carrying the resistance gene Pigm with restoring genes eliminated in the BC2 F2 generation was obtained.After that,the improved line was crossed to Quan 9311 A followed by successive backcrosses,and the CMS line K9311 A and its corresponding maintainer line K9311 B,which were similar to Quan 9311 A in agronomic traits and had a much stronger blast resistance than Quan 9311 A,were successfully developed.

关 键 词：杂交水稻 三系不育系 K9311A 分子标记辅助选择 稻瘟病抗性 Pigm基因 

分 类 号：S511.01[农业科学—作物学] S311