广州地区鲍曼不动杆菌的多位点序列分型及流行克隆  

Multilocus sequence typing and epidemic clones of Acinetobacter baumannii in Guangzhou area

作　　者：郭伟鸿 潘楚芝[2] 黄慕超 方昌全 郭鹏豪[5] 陈惠玲 赵子文 李裕军 GUO Weihong;PAN Chuzhi;Huang Muchao;FANG Changquan;GUO Penghao;CHEN Huiling;ZHAO Ziwen;LI Yujun(Department of Pulmonary and Critical Care Medicine,Guangzhou First People's Hospital,School of Medicine,South China University of Technology,Guangzhou 510180,China;Department of Hepatobiliary Surgery,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China;Department of Pulmonary Medicine,Guangzhou Xinhai Hospital,Guangzhou 510000,China;Department of Pulmonary Medicine,Guangzhou Red Cross Hospital,Guangzhou 510220,China;Department of Clinic Laboratory,The First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China)

机构地区：[1]广州市第一人民医院/华南理工大学附属第二医院呼吸与危重症医学科,广州510180 [2]中山大学附属第三医院肝胆外科,广州510630 [3]广州新海医院呼吸内科,广州510000 [4]广州红十字会医院呼吸内科,广州510220 [5]中山大学附属第一医院检验科,广州510080

出　　处：《广州医药》2022年第1期20-25,共6页Guangzhou Medical Journal

摘　　要：目的本研究对广州地区5家教学医院的鲍曼不动杆菌进行分子流行病学分析。方法5家教学医院共采集138株鲍曼不动杆菌,利用多位点序列分型(multilocus sequence typing,MLST)及eBURST算法评价菌株之间的遗传关系。结果MLST将138株鲍曼不动杆菌分为8个已有序列类型(STs),分别为ST195、ST208、ST457、ST136、ST254、ST548、ST445和ST53,还发现17个新STs。其中ST195的数量最多,占所有分离株的35.5%(49/138),其次为ST208,占所有分离株的21.0%(29/138)。eBURST算法分析显示以ST195为预测祖先型的克隆复合体(clonal complex,CC)195在医院环境中广泛传播。结论鲍曼不动杆菌CC195是广州地区的流行克隆,各家医疗机构应根据其自身实际制定感染防控策略。Objective We analyzed the molecular epidemiology of A.baumannii isolated from 5 teaching hospitals in Guangzhou to identify the epidemic clone in this area.Methods A total of 138 strains of A.baumannii were collected from 5 teaching hospitals,and the genetic relationship was evaluated by multilocus sequence typing(MLST)and eBURST algorithm.Results MLST divided 138 strains of A.baumannii into 8 existing sequence types(STs),namely ST195,ST208,ST457,ST136,ST254,ST548,ST445 and ST53,and 17 new STs.Among them,ST195 had the largest number,accounting for 35.5%(49/138)of all isolates,followed by ST208,accounting for 21.0%(29/138)of all isolates.eBURST algorithm showed that the clonal complex(CC)195,the predicted founder ST195,was widely spread in the hospital environment.Conclusion A.baumannii CC195 was an epidemic clone in Guangzhou area.Medical institution should develop infection prevention and control strategies according to its own actual conditions.

关 键 词：鲍曼不动杆菌 多位点序列分型 广州地区 

分 类 号：R446.5[医药卫生—诊断学]