不同年限毛竹-白及复合系统土壤微生物群落多样性特点  

Soil microbial community diversity in Phyllostachys pubescens-Bletilla striata ecosystems with different intercropping years

作　　者：曹小青 王亮[1] 孙孟瑶 王晶晶[1] 李培玺 储炳银 徐小牛[1] CAO Xiao-qing;WANG Liang;SUN Meng-yao;WANG Jing-jing;LI Pei-xi;CHU Bing-yin;XU Xiao-niu(School of Forestry&Landscape Architecture,Anhui Agricultural University,Hefei Anhui 230036)

机构地区：[1]安徽农业大学林学与园林学院,安徽合肥230036

出　　处：《中国土壤与肥料》2022年第1期147-154,共8页Soil and Fertilizer Sciences in China

基　　金：国家“十三五”重点研发计划(2018YFD0600105);安徽省重点研究和开发计划项目(1804h07020154)。

摘　　要：为了揭示毛竹-白及复合经营系统中植物-土壤间的互作关系,在安徽省广德市邱村镇选择3个不同套种年限(1、3和4年)的毛竹-白及复合林分为研究对象,采用Biolog-ECO微平板技术,结合主成分分析对培养72 h土壤微生物群落功能多样性及其碳代谢特征进行分析。结果表明,毛竹-白及复合林分的土壤微生物碳源平均颜色变化率、Shannon丰富度指数和McIntosh均匀度指数随套种年限的增加呈逐渐升高的趋势,即4年>3年>1年。而Simpson优势度指数没有明显变化。主成分分析显示,3种套种年限的毛竹-白及复合林分土壤微生物群落具有明显的分布差异,且6大类碳源对土壤微生物群落代谢功能均具有一定的影响。土壤有机碳、全磷及pH值与微生物群落多样性指数具有显著相关性(P<0.05),土壤特性是影响土壤微生物群落代谢活性变化的重要因子。随着套种年限的增加,毛竹-白及复合林分的土壤微生物功能多样性逐渐提高。In order to reveal the interaction between plant and soil in an intercropping system of Phyllostachys pubescens Bletilla striata forest,three stands with different intercropping years(1,3,4 a) were selected in Qiucun Town,Guangde City,Anhui Province,and soil microbial community diversity was analyzed using the Biolog-ECO Plate technique based on carbonsource utilization and combining with the principal component analysis. The results showed that the average well color development(AWCD),Shannon richness and McIntosh equality indices of soil microbial community in the intercropping stands of Phyllostachys pubescens-Bletilla striata demonstrated a gradually increasing trend with the increase of intercropping years,i.e.,4 years>3 years>1 year. However,Simpson dominance index had no significant difference among intercropping years. The principal component analysis showed that there were significant differences in soil microbial community distribution among different intercropping years. The six carbon sources influenced the metabolic capacity of soil microbial community to some extent. The soil microbial community diversity indices were significantly correlated with soil organic carbon,total phosphorus,and pH(P<0.05). The results indicated that soil properties were important factors controlling the metabolic activity of soil microbial community. With the increase of intercropping years,soil microbial functional diversity can be gradually improved in Phyllostachys pubescens and Bletilla striata ecosystems.

关 键 词：毛竹 白及 复合模式 土壤微生物 功能多样性 

分 类 号：S714.3[农业科学—林学]