栽培大豆×半野生大豆高密度遗传图谱构建及株高QTL定位  

Construction of a high density genetic map between cultivated and semi-wild soybeans and identification of QTLs for plant height

作　　者：于春淼 张勇[2] 王好让 杨兴勇[2] 董全中[2] 薛红[2] 张明明[2] 李微微[2] 王磊[2] 胡凯凤 谷勇哲 邱丽娟[1,3] YU Chun-Miao;ZHANG Yong;WANG Hao-Rang;YANG Xing-Yong;DONG Quan-Zhong;XUE Hong;ZHANG Ming-Ming;LI Wei-Wei;WANG Lei;HU Kai-Feng;GU Yong-Zhe;QIU Li-Juan(College of Agriculture,Northeast Agricultural University,Harbin 150030,Heilongjiang,China;Keshan Branch of Heilongjiang Academy of Agri-cultural Sciences,Qiqihar 161606,Heilongjiang,China;National Key Facility for Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Utilization,Ministry of Agriculture/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030 [2]黑龙江省农业科学院克山分院,黑龙江齐齐哈尔161606 [3]农作物基因资源与遗传改良国家重大科学工程/农业农村部种质资源利用重点实验室/中国农业科学院作物科学研究所,北京100081

出　　处：《作物学报》2022年第5期1091-1102,共12页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31601326);中央级公益性科研院所基本科研业务费专项(S2021ZD02);中国农业科学院科技创新工程和大豆种质资源保护与利用项目(2019NWB036-05)资助。

摘　　要：采用中SNP160K芯片对丰收24×通交83-611 F2群体252个植株及其亲本进行基因分型,构建了一张由5861个SNP标记组成的全长为3661.46 cM的高密度遗传连锁图谱。利用完备区间作图法(ICIM)定位到7个株高QTL,每个QTL可解释2.56%~10.41%的株高变异。qPH-6-1具有最高的表型变异贡献率和显性效应,可解释10.41%的株高变异,加性效应和显性效应分别为–1.72和18.94;qPH-18-1贡献率次之,可解释9.64%的株高变异,但具有最高的加性效应,达-12.42。在F2群体中筛选出11个qPH-6-1和qPH-18-1基因型为Q6Q6/Q18Q18的单株,平均株高167.00 cm;筛选出16个基因型为q6q6/q18q18的植株,平均株高为91.25 cm。在qPH-18-1定位区间内外增加23个SNP标记,将定位区间由766.97 kb缩小至66.03 kb,包含8个基因,结合基因注释和相对表达量差异分析,推测Glyma.18G279800和Glyma.18G280200可能与大豆的株高相关。本研究为大豆株型的改良提供了分子参考依据和遗传基础。In this study,252 plants and their parents of Fengshou 24×Tongjiao 83-611 were genotyped by SNP160K DNA-chip.A high-density genetic linkage map with a total length of 3661.46 cM was constructed,composing of 5861 SNP markers.Seven QTLs of plant height were detected by inclusive composite interval mapping(ICIM),and each one explained 2.56%-10.41%of the variation in plant height.qPH-6-1 had the highest contribution rate of phenotypic variation and dominant effect,explaining 10.41%of plant height variation,and the additive and dominant effects are–1.72 and 18.94,respectively.The contribution rate of qPH-18-1 was the second,explaining 9.64%of plant height variation,but qPH-18-1 has the highest additive effect,reaching–12.42.11 plants with qPH-6-1 and qPH-18-1 genotypes Q6Q6/Q18Q18 were screened in F2 population with an average plant height of 167.00 cm,and 16 plants with 2-locus genotypes q6q6/q18q18 were screened out with an average plant height of 91.25 cm.The addition of 23 SNP markers inside and outside the qPH-18-1 locus interval narrowed the locus interval from 766.97 kb to 66.03 kb,containing eight genes.Combined with gene annotation and relative expression difference analysis,it was hypothesized that Glyma.18G279800 and Glyma.18G280200 might be associated with plant height of soybean.This study provides a molecular reference base and genetic basis for the improvement of soybean plant architecture.

关 键 词：大豆 株高 SNP 遗传图谱 QTL定位 候选基因 

分 类 号：S565.1[农业科学—作物学]