吉林人参Dof基因家族的鉴定及表达模式分析  

Transcriptome-wide identification and expression pattern analysis for Dof gene family in Panax ginseng from Jilin

作　　者：王康宇[1,2] 郭彦秀 马翀 赵明珠 李凤华[3] 张美萍 王义[1,2] WANG Kang-yu;GUO Yan-xiu;MA Chong;ZHAO Ming-zhu;LI Feng-hua;ZHANG Mei-ping;WANG Yi(College of Life Science,Jilin Agricultural Universily,Changchun 130118,China;Jilin Engineering Research Center for Cinseng Genatic Resources Development and Uilization,Changchun 130118,China;Teaching Department,Heilongjiang Radio and Television University,Harbin 150080,China)

机构地区：[1]吉林农业大学生命科学学院,吉林长春130118 [2]吉林省人参基因资源开发与利用工程研究中心,吉林长春130118 [3]黑龙江广播电视大学教学部,黑龙江哈尔滨150080

出　　处：《中国中药杂志》2022年第1期62-71,共10页China Journal of Chinese Materia Medica

基　　金：国家重点研发计划项目(2013AA102604-3);吉林省科技厅国际合作项目(20200801063GH);吉林省教育厅项目(JJKH20200318KJ,JJKH20200320KJ)。

摘　　要：Dof(DNA binding with one finger)转录因子是植物中特有的一类转录因子,在植物种子发育、组织分化和代谢调控方面起着非常重要的调控作用。为了研究Dof基因家族在人参中的数量和功能,该文以人参转录组数据库为基础,利用生物信息学手段,对人参Dof基因家族成员进行鉴定及其结构、进化、功能分化、表达模式及互作关系等进行了系统分析;同时,将人参Dof基因与已知人参皂苷合成关键酶基因进行关联分析,最终筛选到参与调控人参皂苷生物合成的候选PgDof基因。结果表明,吉林人参Dof基因家族含有57条基因;PgDof基因均具有Zf-Dof保守基序,在进化上比较保守且分成5个组群;表达模式分析结果确定了PgDof基因家族成员在不同组织部位、不同年生和不同农家品种之间存在不同程度的表达差异,同时通过"基因-皂苷含量""基因-基因"连锁分析,获得了PgDof14-1基因是参与调控人参皂苷生物合成的重要候选基因,并建立了该基因的人参发状根遗传转化体系,获得了阳性发状根无性系。该研究为人参中Dof基因家族的研究奠定了理论基础。Dof(DNA binding with one finger),a unique class of transcription factors in plants,play an important role in seed development,tissue differentiation,and metabolic regulation.To identify the number and function of Dof gene family members in Panax ginseng,this study identified the members of Dof gene family in P.ginseng and systematically analyzed their structures,evolution,functional differentiation,expression patterns,and interactions using bioinformatics methods at the transcriptome level.At the same time,the association analysis of Dof genes from P.ginseng with key enzyme genes for ginsenoside synthesis was carried out to screen the candidate PgDof genes involved in the regulation of ginsenoside biosynthesis.The results showed that there were 54 genes belonging to the Dof gene family in P.ginseng from Jilin.All PgDof genes had Zf-Dof conserved motifs,implying that they were evolutionarily conserved and could be divided into five groups.Expression pattern analysis confirmed that the expression of PgDof gene family members in different tissues,different year-old P.ginseng,and different farm varieties varied significantly.Simultaneously,as revealed by"gene-saponin content"and"gene-gene"linkage analysis,an important candidate PgDof14-1 gene involved in the regulation of ginsenoside biosynthesis was obtained.From the established genetic transformation system of this gene in the hairy roots of P.ginseng,a positive hairy root clone was determined.This study has laid a theoretical foundation for the study of Dof gene family in P.ginseng.

关 键 词：人参 Dof基因家族 功能基因 基因表达 

分 类 号：R284[医药卫生—中药学]