稀土氧化钐微粒致大鼠肺损伤的研究  

Study on lung injury induced by rare earth samarium oxide particles in rats

作　　者：赵敖宁 阴海静[2] 范蒙光 张喆 李楠 马腾 Zhao Aoning;Yin Haijing;Fan Mengguang;Zhang Zhe;Li Nan;Ma Teng(Inner Mongolia Medical University,Hohhot 010107,China;School of Public Health,Baotou Medical College,Baotou 014040,China;Center for Comprehensive Disease Control and Prevention of Inner Mongolia Autonomous Region,Hohhot 010020,China)

机构地区：[1]内蒙古医科大学,呼和浩特010107 [2]包头医学院公共卫生学院,014040 [3]内蒙古自治区综合疾病预防控制中心,呼和浩特010020

出　　处：《中华劳动卫生职业病杂志》2021年第12期881-886,共6页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：内蒙古自治区自然科学基金项目(2018MS08048);包头医学院科学研究基金项目(BYJJ-YF20711);包头医学院博士科研启动基金项目(BSJJ201812)。

摘　　要：目的研究三氧化二钐(Sm_(2)O3)微粒对大鼠肺组织的影响,并与相同剂量的二氧化硅(SiO_(2))微粒比较,以期寻找尘肺病的早期筛检参考指标。方法于2018年10月,将72只SPF级健康雄性大鼠以完全随机分组法分为对照组、SiO_(2)组、Sm_(2)O3组,采用一次性非暴露式气管灌注法分别向各组大鼠肺部灌注2.0 ml/kg生理盐水和280 mg/kg的SiO_(2)、Sm_(2)O3微粒悬浮液。用苏木精-伊红(HE)染色法染色大鼠肺脏,观察大鼠肺组织病理改变。采用酶联免疫吸附法检测大鼠血清中SNAIL同源物1(SNAI1)、SNAIL同源物2(SNAI2)、热休克蛋白-27(HSP-27)浓度。取染尘56 d Sm_(2)O3组、对照组大鼠肺组织0.5 g进行长链非编码RNA(lncRNA)、环状RNA(circRNA)筛检。结果染尘7 d时,SiO_(2)组和Sm_(2)O3组大鼠肺泡排列紊乱,围绕支气管壁可见淋巴组织聚集增生;14 d时SiO_(2)组有大量淋巴细胞浸润,Sm_(2)O3组分散存在少量含Sm_(2)O3的巨噬细胞及纤维化结节;28 d时SiO_(2)组出现少量淋巴细胞浸润,Sm_(2)O3组部分区域可见纤维化结节;56 d时SiO_(2)组有少量纤维母细胞增生,Sm_(2)O3组可见大量含灰黑色物质的纤维化结节。不同染尘时间各组大鼠肺脏系数差异均无统计学意义(P>0.05)。染尘14 d时SiO_(2)组大鼠血清中SNAI1、SNAI2含量低于对照组,Sm_(2)O3组血清中SNAI2含量低于对照组,但SNAI1、SNAI2含量高于SiO_(2)组(P<0.05);染尘28 d时SiO_(2)组HSP-27含量低于对照组(P<0.05)。染尘56 d后,Sm_(2)O3组HSP-27含量低于对照组(P<0.05)。染尘56 d后,Sm_(2)O3组lncRNA上调148个、下调725个,circRNA上调16个、下调153个。结论Sm_(2)O3可致大鼠肺损伤,早期即可检测到SNAI2含量的改变,可以作为尘肺病的早期筛检参考指标。大鼠染尘56 d后lncRNA、circRNA表达有差异,可能与尘肺病的发病机制有关。Objective To study the effect of samarium trioxide(Sm_(2)O3)particles on rat lung tissue and compare it with the same dose of silica(SiO_(2))particles,in order to find the reference index for early screening of pneumoconiosis.Methods In October 2018,72 SPF healthy male rats were randomly divided into control group,SiO_(2) group and Sm_(2)O3 group.The lungs of rats in each group were perfused with 2.0 ml/kg normal saline and 280 mg/kg SiO_(2) and Sm_(2)O3 particle suspension by one-time non exposed tracheal perfusion.The lungs of rats were stained with hematoxylin eosin(HE)staining,and the pathological changes of lung tissues were observed.The concentrations of SNAIL homologue 1(SNAI1),SNAIL homologue 2(SNAI2),and heat shock protein-27(HSP-27)in rat serum were detected by enzyme-linked immunosorbent assay.0.5 g of lung tissue from rats in Sm_(2)O3 group and control group exposed to dust for 56 days was screened for long-chain noncoding RNA(lncRNA)and circular RNA(circRNA).Results After 7 days of dust exposure,the alveoli in SiO_(2) group and Sm_(2)O3 group were disordered,and lymphoid tissue aggregation and proliferation were observed around the bronchial wall.At 14 days,a large number of lymphocytes infiltrated in SiO_(2) group,and a small number of macrophages containing Sm_(2)O3 and fibrotic nodules scattered in Sm_(2)O3 group.At 28 days,a small amount of lymphocyte infiltration appeared in SiO_(2) group,and fibrotic nodules were seen in some areas of Sm_(2)O3 group.At 56 days,there was a small amount of fibroblast proliferation in SiO_(2) group,and a large number of fibrotic nodules containing gray black matter were seen in Sm_(2)O3 group.There was no significant difference in lung organ coefficient among groups at different dust exposure time(P>0.05).After 14 days of dust exposure,the contents of SNAI1 and SNAI2 in serum of rats in SiO_(2) group were lower than those in control group,the content of SNAI2 in serum of Sm_(2)O3 group was lower than that in control group,and the contents of SNAI1 and SNAI2 in ser

关 键 词：大鼠 肺损伤 三氧化二钐 二氧化硅 SNAIL同源物1 SNAIL同源物2 热休克蛋白质类 长链非编码RNA 环状RNA 

分 类 号：R135.2[医药卫生—劳动卫生]