耐火陶瓷纤维致大鼠肺损伤及血清克拉拉细胞蛋白16和表面活性蛋白D水平变化  

Refractory ceramic fibers induced changes in serum Clara cell protein 16 and surfactant protein D levels in rats

作　　者：杨思雯 阎腾龙 朱晓俊 马文军[1] Yang Siwen;Yan Tenglong;Zhu Xiaojun;Ma Wenjun(School of Public Health,Peking University,Beijing 100191,China;Beijing Institude of Occupational Disease Prevention and Treatment,Beijing 100093,China;National Center for Occupational Safety and Health,National Health Commission of the People's Republic of China,Beijing 102308,China)

机构地区：[1]北京大学公共卫生学院,100191 [2]北京市职业病防治研究院,100093 [3]国家卫生健康委职业安全卫生研究中心,北京102308

出　　处：《中华劳动卫生职业病杂志》2021年第12期887-892,共6页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：北京市自然科学基金(7192137)。

摘　　要：目的探讨耐火陶瓷纤维(RCFs)对Wistar大鼠血清克拉拉细胞蛋白16(CC16)和表面活性蛋白D(SP-D)水平的影响。方法于2020年10月,将96只健康成年雄性Wistar大鼠随机分为对照组(等体积生理盐水)、低剂量组(5 mg/ml RCFs)、中剂量组(10 mg/ml RCFs)和高剂量组(20 mg/ml RCFs),进行非暴露式气管滴注染毒,大鼠经腹腔麻醉后,滴注RCFs混悬液或生理盐水200μl,每隔3天一次,共4次。分别于染毒后7、14、28和90 d经腹主动脉取血处死6只大鼠,分离其脏器,观察肺组织病理学改变并进行肺损伤评分,酶联免疫吸附测定(ELISA)法测定血清CC16、SP-D含量。结果RCFs染毒可导致大鼠肺组织炎症细胞浸润、肺间隔增宽、肺泡结构破坏等炎性损伤。染毒后7 d,各剂量组大鼠肺损伤评分高于对照组,高剂量组肺损伤评分高于低剂量组(P<0.05);染毒后14、90 d,中、高剂量组大鼠肺损伤评分高于对照组(P<0.05);染毒度后28 d,高剂量组肺损伤评分高于对照组(P<0.05)。染毒后7 d,中、高剂量组大鼠血清CC16和SP-D浓度明显高于对照组和低剂量组(P<0.05);染毒后28 d,低、中剂量组大鼠血清CC16浓度明显低于对照组和高剂量组(P<0.05);染毒后90 d,大鼠血清CC16浓度随染毒剂量的升高而降低(F=28.853,P<0.01),SP-D浓度随染毒剂量的升高而升高(F=25.636,P<0.01)。结论RCFs染毒可能对大鼠克拉拉细胞、肺泡-毛细血管屏障造成一定的损伤,可通过血清CC16和SP-D的动态变化间接了解肺损伤的严重程度。Objective To explore the effect of refractory ceramic fibers(RCFs)on the serum Clara cell protein 16(CC16)and surfactant protein D(SP-D)levels in Wistar rats.Methods In October 2020,96 healthy adult male Wistar rats were randomly divided into control group(equal volume of normal saline),low-dose group(5 mg/ml RCFs),medium-dose group(10 mg/ml RCFs)and high-dose group(20 mg/ml RCFs),and subjected to non-exposure tracheal instillation.After intraperitoneal anesthesia,the rats were instilled with 200μl of RCFs suspension or normal saline,once every 3 days for a total of 4 times.At 7,14,28,and 90 days after exposure,6 rats were sacrificed by blood sampling through the abdominal aorta.The organs were separated,histopathological changes of lungs were observed and lung injury scores were performed.The contents of serum CC16 and SP-D were determined by enzyme-linked immunosorbent assay(ELISA).Results RCFs could cause inflammatory cells in rat lung tissues,widening of the lung septum and destruction of alveolar structure.7 days after exposure,the lung injury scores of rats in each dose group were higher than control group,and the lung injury score of the high-dose group was higher than low-dose group(P<0.05).14 and 90 days after exposure,the lung injury scores of the medium-dose and high-dose groups were higher than control group(P<0.05).28 days after exposure,the lung injury score of the high-dose group was higher than control group(P<0.05).7 days after exposure,the serum CC16 and SP-D concentrations of rats in the medium-dose and high-dose groups were significantly higher than control and low-dose groups(P<0.05).28 days after exposure,the serum CC16 concentrations of rats in the low-dose and medium-dose groups were significantly lower than those of the control and high-dose groups(P<0.05).After 90 days of exposure,the serum CC16 concentrations of rats decreased with the increase of the exposure dose(F=28.853,P<0.01),and the concentrations of SP-D increased with the increase of the exposure dose(F=25.636,P<0.01).Conclusi

关 键 词：大鼠 耐火陶瓷纤维 克拉拉细胞蛋白16 表面活性蛋白D 

分 类 号：R135[医药卫生—劳动卫生]