基于过氧化氢酶介导金纳米颗粒交联聚集的乙肝表面抗原可视化检测  

Naked-eye Detection of Hepatitis B Surface Antigen Based on Catalase-Mediated Crosslinking Aggregation of Gold Nanoparticles

作　　者：陈如冰 胡永琴 陈美珠 安佳 吕颖 刘玉菲[1,2] 李东玲[1] CHEN Rubing;HU Yongqin;CHEN Meizhu;AN Jia;LYU Ying;LIU Yufei;LI Dongling(Key Laboratory of Optoelectronic Technology&Systems,Ministry of Education,Chongqing University,Chongqing 400044,China;Center for Intelligent Sensing Technology(CIST),College of Optoelectronic Engineering,Chongqing University,Chongqing 400044,China)

机构地区：[1]重庆大学光电技术及系统教育部重点实验室,重庆400044 [2]重庆大学光电工程学院智能感知技术中心(CIST),重庆400044

出　　处：《材料导报》2022年第5期118-121,共4页Materials Reports

基　　金：中央高校基本科研业务费专项资金(2019CDQYGD020,2019CDCGGD304);国家重点研发开发项目(2016YFE0125200);国家自然科学基金(61927818,62005030)。

摘　　要：开发高效、高灵敏度又适用于基层的简便、稳定、成本低的病毒标志物检测方法对病毒的早期诊断具有重要意义。本工作通过制备同时具有催化和特异性识别能力的双功能聚苯乙烯纳米球催化H_(2)O_(2)分解,剩余的H_(2)O_(2)通过交联剂诱导金纳米颗粒聚集,使溶液颜色从红色变为蓝色,实现对乙肝表面抗原的高效、高灵敏、高特异性可视化检测。肉眼检测限为0.5 ng/mL,借助酶标仪后的检测限为0.01 ng/mL。此外,HBsAg在血清中的回收率在88.17%~102.50%,相对标准偏差在2.01%~4.52%,表明所提出的免疫测定法适用于血清中的检测,在实际临床中具有广阔的应用前景。Developing efficient,highly sensitive,simple,stable,and low-cost virus markers detection productsfor local clinics is of great emphasis in the early diagnosis and treatment of the virus.In this paper,the bifunctional polystyrene nanospheres with both catalytic and specific recognition capabilities are prepared to catalyze the decomposition of H_(2)O_(2),and the remaining H_(2)O_(2) will induce the aggregation of gold nanoparticles through the crosslinking agents,which causes the color of the solution to change from red to blue,so as to achieve the high-efficiency,high-sensitivity and high-specificity naked-eye detection of hepatitis B surface antigen.The detection limit of the naked-eye was recorded as 0.5 ng/mL and the detection limit assisted with microplate reader is 0.01 ng/mL.In addition,the recovery rate of HBsAg in serum is between 88.17%—102.50%,and the relative standard deviation is between 2.01%—4.52%,indicating that the proposed immunoassay is suitable for the detection in serum and has great potential in actual clinical practice.

关 键 词：可视化检测 乙肝表面抗原 催化 金纳米颗粒 聚集 

分 类 号：Q55[生物学—生物化学]